collagenase

collagenase

1. The HUVECs were isolated from fresh umbilical vein with 0.1% collagenase A and were identified by cell morphology and immunostaining.

0.1%胶原酶灌注获取脐静脉内皮细胞，光镜和免疫组织化学鉴定。

2. L-1cartopril eye drops has stronger efficacy to inhibit collagenase, with less side effect or stimulation，more convenient resource and preparation than other eye drops.

L-1卡托普利眼液抑制胶原酶能力极强、副作用小、刺激性极微、药物来源及配制方便。

3. MPS inhibits various aggressive lysosomal enzymes that degrade important constituents of connective tissue, such as elastase (degradation of elastin), collagenase (degradation of collagen) and hyaluronidase (degradation of hyaluronic acid).

MPS抑制了各种参与分解代谢的酶，这些酶用于降解结缔组织的重要组成元素，如弹性蛋白酶（降解弹性蛋白），胶原酶（降解胶原）和透明质酸酶（降解透明质酸）。

4. Effectiveness and Safety of Different Injection Sites of Collagenase for Lumbar Intervertebral Disc Protrusion: A Systematic Review

不同部位和途径注射胶原酶治疗腰椎间盘突出症有效性与安全性的系统评价

5. It attracts much attention in clinic that whether there is nerve injury induced by collagenase chemonucleolysis (CCN) around the injection sites.

临床最关心的是胶原酶对注射部位周围的神经是否有损伤。

6. In order to overcome the immunogenicity of collagenase,the enzyme was modified with monomethoxypolyethylene glycol 5000 (mPEG) activated by p nitrophenyl chloroformate.

为解决胶原酶免疫原性，采用经氯甲酸对硝基苯酯活化的单甲氧基聚乙二醇来修饰胶原酶。

7. The clinical study of twice chemonucleolysis with collagenase for the treatment of lumbar disc herniation

二次注射胶原酶治疗腰椎间盘突出症的临床研究

8. Keywords gallnut water extract;periodontal inflammation;Collagenase;

五倍子水提取物;牙周炎;胶原酶;

9. Isolation and purification of angiotensin converting enzyme inhibitor from pig bone collagenase hydrolysate using affinity chromatography

亲和色谱法从猪骨胶原蛋白酶解物中分离纯化血管紧张素转换酶抑制剂

10. Observation of collagenase activity in hypertrophic scar and normal skin in burned patient

人体正常皮肤及增生性瘢痕胶原酶活性的检测

11. Keywords Human augmentor of liver regeneration;Hepatic cell;Collagenase;Gene expression;

人肝再生增强因子;肝细胞;胶原酶;基因表达;

12. THE USE OF SYRINGE PUMP DURING LUMBAR DISCS HERNIATION TREATED BY INJECTION OF COLLAGENASE

佳士比注射泵注入胶原酶治疗腰椎间盘突出症

13. Localization for collagenase injection through the lateral crypt for lumbar disc herniation

侧隐窝注射胶原酶治疗腰椎间盘突出症定位方法探讨

14. Tbe Observation of Treatment Results of Lumbar Disc Herniation Using Chemonucleolysis with Extra Disc Injection of Collagenase

侧隐窝穿刺置管胶原酶溶盘的疗效观察

15. immunoreactive human skin collagenase

免疫反应性人皮肤胶原酶

16. Variation of collagenase content in lacrima after cornea alkali burn in rabbit model

兔角膜碱烧伤后泪液中胶原酶含量变化

17. Keywords chemonucleolysis;collagenase;intervertebral disk displacement;CT number;interventional therapy;

关键词化学溶解术;胶原酶;椎间盘移位;CT值;介入治疗;

18. Keywords Collagenase;Lumbar intervertebral disc protrusion;Interior and exterior injection;Extradural injection;

关键词胶原酶;腰椎间盘突出症;突出物内外联合注射;硬膜外注射;

19. An anti-oxidation function restricts the formation of collagenase and restores the strength of skin.

具抗氧化功效，对抗肌肤真皮层中胶原蛋白硬化，令肌肤重拾弹性。

20. Keywords endothelium/cytology;interstitial collagenase;estradiol;high glucose;reverse transcriptase polymerase chain reaction;apoptosis;

内皮/细胞学;间质胶原酶;雌二醇;高糖;逆转录聚合酶链反应;细胞凋亡;

21. Keywords Arteriosclerosis;Growth substances;Interstitial collagenase;Drug therapy;

动脉硬化;生长物质;间质胶原酶;药物疗法;

22. Keywords Chemonucleolysis;Collagenase;Inflammation;Intervertebral disk displacement;Phos-pholipase A2;Rat;

化学髓核溶解术;胶原酶;炎症;椎间盘移位;磷脂酶A2;大鼠;

23. Comparison between the effects of simplex collagenase chemonucleolysis and its combination therapy with automated percutaneous lumbar discectomy on lumbar disc herniation

单纯胶原酶与联合切吸治疗腰椎间盘突出症的疗效对比

24. Utilizing the no-vascular interventional procedure under fluoroscopic guidance, patients underwent performnace of CNL and PLDD, had disc vaporized with laser and were injected collagenase.

在X线透视下，采用非血管性介入治疗技术，应用专门的特殊器械，经皮穿刺至椎间盘及椎间孔的相应位置，分别进行激光汽化椎间盘及注射胶原蛋白溶解酶。

25. The experimental study of the effect of dexamethasone, lidocaine and contrast medium on the activity of collagenase

地塞米松、利多卡因、对比剂对胶原酶活性影响的实验研究

26. Gene expression of interstitial collagenase MMP-13 in progressive phase of rat liver fibrosis induced by ethanol

基质金属蛋白酶-13在大鼠酒精性肝纤维化表达的研究

27. PROLAPSE OF LUMBAR INTERVERTEBRAL DISK TREATED WITH POSITIONAL IMPROVED LATERAL CRYPT COLLAGENASE INJECTION

定位改良侧隐窝注射胶原酶治疗腰椎间盘突出症

28. Experimental electromyography of collagenase chemonucleolysis out of lumbar intervertebral disc

实验性胶原酶盘外溶核的肌电图分析

29. Observation of Collagenase Activity in Intervertebral Disc after Experimental Dynamic Nonequilibrium of Cervical Vertebrae

实验性颈椎动力平衡失调后椎间盘胶原酶活性观察

30. Some doubts about the article "analysis of fever after injection of collagenase for the treatment of lumbar disc herniation"

对"注射胶原酶治疗腰椎间盘突出症术后发热分析"一文的质疑

31. The dermal papillae were released synchronously by blowing up the collagenase with pipette.

对其贴壁率、细胞迁出率、工作强度、污染机会与显微解剖法、显微解剖加酶消化法进行比较。

32. Keywords Vacuum_assisted closure（VAC）;Chronic wound;Collagenase;

封闭负压引流;慢性创面;胶原酶;

33. We considered CT number of the hernial disc as an objective index for prognosis of collagenase chemonucleolysis.

我们提出用CT值量化突出椎间盘的硬度,预测胶原酶溶解术后疗效。

34. Abstract A method of isolating P cells of rat sinoatrial node by collagenase is described.

摘要 介绍一种用胶原酶分离和初步鉴定大鼠窦房结起搏细胞的方法。

35. Objective To discuss the problems of treating for lumbar disc herniation with collagenase chemonucleolysis and microendoscopic discectomy.And give the help for clinic.

摘要目的讨论注射法胶原酶化学溶解术和椎间盘镜髓核摘除两种微创技术治疗腰椎间盘突出症的有关问题，旨在为临床工作提供指导。

36. Objective To evaluate the therapeutic effects of collagenase chemonucleolysis injection on lumbar intervertebral disc extrusion by percutaneous putamen in CT position.

摘要目的评价CT引导下经皮硬膜外靶位注射胶原酶治疗腰椎间盘脱出的作用及效果。

37. Methods Animal model was established by injecting collagenase into left caudoputamen of adult rats under stereotaxic apparatus.

方法 大鼠尾壳核注射胶原酶造成脑出血模型。

38. METHODS Portal vein cannulation and perfusion combined with collagenase digestion were used to isolate pig pup hepatocytes.They were then cultured and passaged into the conditioned medium.

方法 用门静脉插管原位灌注结合胶原酶消化法分离乳猪肝细胞,应用条件培养基对其进行体外培养,观察其形态、增殖、分化和功能等方面的变化.

39. Methods The islets of NMRI mice were isolated by digestion combined with collagenase and DNase, incubated in RPMI 1640 tissue culture medium overnight. The experiments were completed with Millipore Multiscreen Assay System.

方法 胶原酶和DNA酶联合消化法分离NMRI小鼠胰岛 ,RPMI1 640组织培养液过夜培养 ,采用MilliporeMultiscreen系统观察CDS对胰岛功能的影响。

40. Methods The SD rats islets were isolated by digestive method with collagenase and DNase. Millipore Multiscreen Assay System was used to observe the effects of phentolamine on the function of isolated islets.

方法 胶原酶和DNA酶联合消化法分离SD大鼠胰岛 ,RP MI164 0组织培养液过夜培养 ,采用MilliporeMultiscreen系统观察不同浓度葡萄糖及酚妥拉明对胰岛功能的影响。

41. Methods The islets were got from SD rats by collagenase digestion. Millipore Multiscreen System was used to observe the effects of imidazoline compounds (phentolamine, efaroxan and RX871024) on the function of isolated islets.

方法 胶原酶和DNA酶联合消化法分离SD大鼠胰岛 ,采用MilliporeMultiscreen系统观察不同葡萄糖 (Glu)浓度及咪唑啉类化合物酚妥拉明、Efaroxan和RX8710 2 4对胰岛功能的影响。

42. Methods The sciatic and brachial plexus nerve of three-day-old SD mice was treated with collagenase type IV and trypsin to remove the desmocyte on the Schwann cell, then explanted in the culture disk.

方法:先用IV型胶原酶和胰蛋白酶消化出生3天的SD鼠臂层神经和坐骨神经组织块后再将组织块贴壁培养。

43. Methods: 25 cases of ocular burn patients were treated with local and systemic corticosteroids, vasodilator and collagenase inhibitor.

方法:对25例中度重眼烧伤患者行局部及系统皮质类固醇及激素治疗和扩血管药物、胶原酶抑制剂治疗。

44. METHODS:The islets were isolated from NMRI mice by collagenase and DNase digestion. Millipore multiscreen assay system was used to observe the effects of PACAP 38 on the function of isolated islets.

方法:用胶原酶和DNA酶联合消化法分离NMRI小鼠胰岛,RPMI1640组织培养液过夜培养,采用MilliporeMultiscreen系统观察不同浓度PACAP-38对胰岛功能的影响。

45. Methods: Normal endometrial tissues were isolated and cultured in vitro by double collagenase digestion and selective wall-pasting.

方法:选择正常子宫内膜组织,通过消化、过滤、选择性贴壁和二次消化等技术进行体外培养。

46. Methods: Osteoblasts were isolated from neonatal r at calvaria through trypsin and collagenase digestion.

方法:酶消化法分离培养新生大鼠颅盖骨成骨细胞；

47. Methods:8 goats were used and TMJOA was induced by injection of collagenase in upper joint spaces. 4 weeks after injection, the left joints were treated with NG-monomethyl-arginine (L-NMMA).

方法:采用山羊作为实验动物,以关节上腔内注射胶原酶制造TMJOA动物模型,制造模型后第4周起,在左侧关节注射L-NMMA,右侧注射生理盐水作为对照。

48. Methods:In this experiment,the rat model of intracranial hemorrhage was made with bacterial collagenase,which was infused into the right candate nucleus.

方法:采用胶原酶尾状核注射法造模,观察安宫牛黄丸原方及简方对脑出血损伤大鼠脑组织匀浆中超氧化物歧化酶(SOD)、丙二醛(MDA)含量的影响。

49. Methods:Collagenase solution was injected through the pancreatic duct.The splenic lobe of porcine pancreas was digested by static digestion combined with physics digestion.

方法:采用胶原酶胰导管注射负荷技术,静止消化与物理消化相结合消化分离成年猪胰腺脾叶;

50. Methods We collected 32 cases with lumber intervertebral disc extrusion diagnosed by clinic and examined CT or(and)MRI within 3 months, who underwent percutaneous collagenase chemonucleolysis.

方法CT引导下经皮穿刺硬膜外靶位注射胶原酶治疗临床诊为腰椎间盘脱出并经CT和/或MRI检查(3个月内)证实的患者32例。

51. Methods We collected 32 cases with lumber intervertebral disc extrusion diagnosed by clinic and examined CT or (and) MRI within 3 months, who underwent percutaneous collagenase chemonucleolysis.

方法CT引导下经皮穿刺硬膜外靶位注射胶原酶治疗临床诊为腰椎间盘脱出并经CT和／或MRI检查（3个月内）证实的患者32例。

52. Methods Rabbit cardiac myocytes were isolated with low calcium, protease and collagenase successively and the myocytes were cultured with suspending and adherent method.

方法依次用低钙、蛋白酶及胶原酶灌流兔心脏,分离心肌细胞,并用悬浮法和贴壁法进行无血清原代培养。

53. Methods Sixty patients with herniated lumardisk underwent CT guided combined injection of O3 and collagenase,which comprised intradisk and itraprocessus injection.

方法在CT引导下对60例腰椎间盘突出症患者,采用盘内注射以及椎体后路突出物内注射臭氧(O3)及胶原酶来治疗腰椎间盘突出症。

54. Methods To incubate the caries free permanent teeth with acid gelatinoid and/or collagenase, then to observe the ultrastructural change of root surface under scanning electron microscope .

方法将无龋恒牙分组经酸性胶液和胶原酶分别或共同孵化处理后切割制样,脱水干燥,喷金,扫描电镜观察根面超微结构的变化。

55. Methods The expression and activity of collagenase in hypertrophic scar and normal skin were studied with in situ hybridization and SDS-polyacrylamide gel electrophoresis.

方法应用原位杂交和SDS-聚丙烯酰胺凝胶加胶原底物电泳观测了肥厚性瘢痕组织中胶原酶表达、酶活性及胶原的降解。

56. Methods Alveolar macrophages recovered by lung lavage and interstitial macrophages by treatment of the lung tissue with DNAse and collagenase,the AM were treated in the same way as IM.

方法支气管肺泡灌洗获取肺泡巨噬细胞,肺组织机械处理结合酶消化法(DNA酶、胶原酶)分离间质巨噬细胞;

57. Method Rat islets were isolated with in situ ductal collagenase distention, stationary digestion and dextran gradient separation.

方法本实验经胰管注射胶原酶、胰腺静止消化分离成年大鼠胰岛,葡聚糖离心纯化,获得胰岛细胞,进行记数和功能鉴定。

58. Methods The collagenase inhibitor’s effects were detected by rat's collagen and collagenase with comassie brilliant blue colorimetric method.

方法用鼠胶原、胶原酶及考马斯亮蓝比色法来检测药物抑制胶原酶的能力。

59. Methods The islets were isolated and purificated by collagenase perfusion method,and counted by dithizone staining.The standard of isolated live cells was justified by trypan blue staining.

方法通过用胶原酶灌注分离纯化胰岛,用双硫棕染色,台盼蓝染色和胰岛素释放试验来鉴定胰岛的数量、形态和功能。

60. Methods Using different techniques of collagenase digestion and discontinued density gradient centrifugation, the adult swine and rat islets were prepared.

方法采用不同的胶原酶消化法及不连续密度梯度纯化法。

61. Methods The human choroidal tissues were digested in two steps by trypsin and collagenase, and the HCEC were obtained and cultured after the digested cell suspension was sorted and purified with magnetic beads of CD31 Dynabeads.

方法采用胰酶、胶原酶对人眼脉络膜组织块进行两步消化,应用免疫磁珠CD31 Dynabeads对消化后的细胞混合悬液分选纯化后获得人眼脉络膜血管内皮细胞并进行细胞培养;

62. Methods The esophageal carcinoma cell line CSEC was established by collagenase digestion methods.

方法采用胶原酶消化法建立人食管癌细胞系CSEC。

63. Methods: The upper compartments of both TMJs of 8 goats were injected with collagenase to induce osteoarthrosis.

方法：对8只山羊的双侧颞下颌关节上腔一次性注射胶原酶而诱导骨关节病病变。

64. Methods: Endometrial glandular cells and stromal cells were isolated by collagenase digestion and copper sieve filtration methods and treated with monolayer cultured.

方法：用胶原酶消化和铜网过筛法分离子宫内膜基质细胞和上皮细胞，进行单层培养。

65. Comparison of the clinical effect between single microendoscopic discectomy and its combining with collagenase chemonucleolysis for treatment of lumbar disc herniation

显微内窥镜下椎间盘切除术及其与胶原酶溶核术联合治疗腰椎间盘突出症的疗效比较

66. Bromley J W,Intervertebral discolysis with collagenase[J]．Drug Res,1982,32(11)：1045.

杨述华，杜靖远，罗怀灿，等．化学溶解术治疗椎间盘突出症的临床研究[J]．中华骨科杂志，1996，16：415

67. Clinical Study on Treatment of Prolapse of Lumbar Intervertebral Disc by Injection of Collagenase through Lateral Angles of Canalis Vertebralis

椎管外侧角注射胶原酶治疗腰椎间盘突出症的临床研究

68. Keywords Intervertebral disk chemolysis;Neural conduction;Collagenase;

椎间盘化学松解术;神经传导;胶原酶类;

69. Keywords intervertebral disk chemolysis;collagenase;intervertebral disk herniat ion;target-injection method;

椎间盘化学溶解术;胶原酶;椎间盘突出症;靶位注射法;

70. Keywords Intervertebral disk displacement;Diskoscope;Collagenase;

椎间盘移位;椎间盘镜;胶原酶;

71. Keywords Intervertebral disk displacement;Collagenase;

椎间盘移位;胶原酶;

72. Keywords intervertebral disc herniation;lumbar diskectomy;collagenase;chemonuleolysis;

椎间盘突出;椎间盘切吸术;胶原酶;溶解术;

73. Keywords Computed tomography;Lumbar disc herniation;Collagenase;Intervertebral disk chemolysis;

椎间盘突出症;胶原酶;椎间盘化学松解术;

74. Clostridium histolyticum collagenase

溶组织梭菌胶原酶

75. Isolation and Characterization of Collagenase From Clostridium Histolyticum

溶组织梭菌胶原酶的提取及活性分析

76. The patients in PLDD group were treated by PLDD using Nd:YAG successive laser with 1 064- nm wave length,and those in CNL group were treated with CNL by slowly injecting 1 200 U collagenase dissolved in 5 mL normal saline.

激光椎间盘减压术组采用激光椎间盘减压术介入治疗,Nd:YAG连续型激光,波长1064nm; 胶原酶溶核术组采用胶原酶溶核术治疗,将胶原蛋白溶解酶1200U溶于5mL生理盐水中,缓慢注入。

77. Keywords Burns;Clostridium histolyticum collagenase;Treatment outcome;

烧伤;溶组织梭菌胶原酶;治疗结果;

78. Model of intracerebral hematoma induced by collagenase injection by stereotatic technique in cats

猫脑内立体定向注射胶原酶建立脑内血肿模型

79. Canalis E,Rydzliel S,lklang A,et al.Insulin-like growth factors inhibit interstitial collagenase synthesis in bone cell cultures.Endocrinology,1995,136:1348.

王敏,辛海变,黄象艳.胰岛素样生长因子-I对成骨细胞生长影响的实验研究.中国矫形外科杂志,2004,3(4):236.

80. Effects of diammonium glycyrrhizinate on the lipid peroxidation and interstitial collagenase activity in fibrotic liver in rats

甘草酸二铵对肝纤维化大鼠肝脏脂质过氧化与间质性胶原酶活性的影响