cytometry

cytometry

1. Cell circle stage was measured by flow cytometry assays after incubation of 10 U/ml EP for 72 h.

10U/mlEP与MSCs共培养72h后,流式细胞仪检测细胞周期情况。

2. Flow cytometry using CFSE labeling is reliable,sensitive,precise and convenient for apoptotic cell tracing in vivo and in vitro.

CFSE标记细胞后诱导凋亡,再经流式细胞术检测组织内细胞分布,必要时辅以组织学检测可以作为一种可靠、高效、经济的凋亡细胞体内示踪研究技术方法。

3. The application of automated image cytometry in sputum cytologic diagnosis of lung cancer 1.

DNA aneuploidy for diagnosis of lung cancer (全自动图象细胞仪在肺癌痰细胞学诊断中的应用1.

4. The value of DNA ploidy analysis by flow cytometry in diagnosis of pleural effusion and the ascitic cells.

DNA倍体分析在胸腹水诊断中的价值。

5. FAM-de coy ODNs were used as control, and transfected into K562 cells by cationic lipos omes, analyzed by flow cytometry (FCM) and fluorescent inversive microscopy.

FAM鄄decoyODNs作为对照,在脂质体介导下转染白血病细胞K562,流式细胞仪(FCM)和倒置荧光显微镜检测FAM鄄decoyODNs的摄入情况。

6. The expression of B-RAF gene and Bcl-2 gene in BGC823 cells was detected by RT-PCR and the level of apoptosis was evaluated in transfected cells by flow cytometry.

MTT检测胃癌BGC823细胞增殖情况; 流式细胞仪检测细胞凋亡情况,并与对照组进行比较。

7. Flow cytometry showed the cells in G2 phase were mainly increased(P<0.01).

MTT法显示细胞增殖高于对照组,细胞周期检测示实验组G2期细胞高于阴性对照组,P<0.01。

8. The IC_ 50 of daunorubicin (DNR) in Jurkat cells was examined by MTT and the concentration and distribution of DNR in Jurkat cells were detected by flow cytometry.

MTT法测定柔红霉素(daunorubicin,DNR)在Jurkat细胞中的IC50及运用流式细胞仪检测DNR在Jurkat细胞内的储积浓度及分布;

9. NK cell frequencies were analyzed using flow cytometry.

NK细胞频率采用流式细胞仪进行分析。

10. An evaluation of NMP 22 urinary level and flow cytometry for diagnosing bladder transitional cell carcinoma.

NMP 22与流式细胞术在膀胱癌诊断中的价值

11. The apoptotic percentage of UVC-treated-SL-1 cells was measured by flow cytometry using propidium iodide( PI) staining.

PI染色法检测其凋亡率,处理后8hr的凋亡率为47.08%。

12. The expresses of Bcl-2 and Bax in cell linesCOC1 and COC1/DDP (before and after the treatment of mifepristone) were detemined by flow cytometry (FCM).

不同浓度的mifepristone对耐药细胞株COC1/DDP Bc1-2和Bax表达的影响。

13. Flow cytometry was used to study the effect on cell cycle by AraC alone and in combination with EGCG.

以流式细胞光度仪分析联合用药前后对细胞周期和细胞凋亡的影响。

14. When CBT cells were treated with low temperature for 3days,flow cytometry revealed the hypodiploid apex and the apoptotic rate was shown to be 13%.

低温处理3 d的CBT细胞经流式细胞仪检测出现亚二倍体峰,凋亡率为13%.

15. GRC-1 RCC cell line was cultured with the addition of 5-FU or adriamcin (ADR) and the GRC-1 RCC cell apoptosis was assayed by flow cytometry (FCM).

体外GRC - 1肾癌细胞株与化疗药物 5 -氟尿嘧啶 (5 -FU)、阿霉素共同培养 ,流式细胞仪 (FCM)检测肾癌细胞株的凋亡。

16. The expressions of CD55 and CD59 on peripheral granulocytes and erythrocytes were analyzed with flow cytometry.

健康对照组20名。以流式细胞术检测外周血粒、红细胞CD55、CD59的表达。

17. MACS assay was used to purify CD34+ cells from MNC, the purity of the CD34+ cells was evaluated by flow cytometry.

免疫磁分选技术纯化人脐血CD34十细胞，流式细胞术鉴定纯度;

18. Immunocytochemistry and flow cytometry analysis showed that almost all of the adherent cells were vWF positive and around 90% were able to bind UEA I specifically.

免疫细胞化学和流式细胞术的结果显示 ,几乎所有贴壁细胞为vWF阳性 ,且约 90%的细胞具有UEA I结合能力。

19. Title： The value of DNA ploidy analysis by flow cytometry in diagnosis of malignant pleural effusion.

关键词：流式细胞术;癌胚抗原;脱落细胞学;恶性胸腔积液

20. The main methods including flow cytometry(FCM) and image cytophotometry(ICM).

其主要测定方法有流式细胞术和细胞图像光度术。

21. Erythrocyte associated IgG,A and M were measured by a flow cytometry (FCM).

利用流式细胞术和抗人IgG单克隆抗体检测红细胞表面抗体IgG。

22. Fluorescent microspheres are applied in flow cytometry assay of epidemic hemorrhagic fever.

发展了一种应用荧光微球进行流行性出血热流式细胞术检测的新方法。

23. Apoptosis of thymocytes of these mice was assayed by flow cytometry.

取胸腺细胞,通过流式细胞仪计数,检测胸腺细胞凋亡率。

24. The enhanced green fluorescent protein(EGFP) plasmid was used as positive control and the transfection efficiency was detected by flow cytometry.

同时转染带有增强型绿色荧光蛋白(EGFP)的载体作为阳性对照,流式细胞术检测其绿色荧光以确定转染效率;

25. Pictured here is the flow cytometry pattern of a breast carcinoma.

图示：乳腺癌流式细胞仪模式图。

26. Flow cytometry provides a quick and objective technique to assess cell fusion for nuclear reprogramming studies.

在细胞核的程序性重编的研究中，流式细胞术对评估细胞融合来说是一项既快捷又极具客观性的技术。

27. The cell apoptotic rate was measured by flow cytometry (FCM) and Hoechst 33258 DNA staining method.

姜酚化学性质活泼，接触酸、碱、热易发生变性，不易保存。

28. The infected cells were analyzed by flow cytometry with monoclonal antibodies specific for EV71.The virus titer was quantitated by plaque assay.

将采集的周边血液单核球细胞或是脐带血液单核球细胞利用梯度离心分离出来后，再以肠病毒71感染。

29. The dynamic relationship between cytotoxic effect and incubated time with TPT was investigated by flow cytometry and immunological analysis.

应用新的流体细胞测量仪(FACS-Vantage)及免疫分析法对细胞培养时间、TPT的浓度与拓扑酶中毒程度的动力学关系进行了研究。

30. Flow cytometry was used to determine the influence of blockade of CLC-3 on cell cycle.

应用流式细胞仪观察CLC一3反义核酸对细胞周期的影

31. The flow cytometry (FCM) was used to investigate the phase of the proliferative cycles of Hela cells.

应用流式细胞仪（FCM）观测米非司酮对细胞周期分布的影响；

32. The effects of CpG-N ODN on the surface binding and internalization of CpG-S ODN were tested by flow cytometry.

应用流式细胞术观察CpG-NODN对CpG-S ODN与hPBMC表面结合和内化的影响。

33. Electron microscopy and flow cytometry were used to demonstrate apoptotic changes in AS ODN treated cells. Results Both AS ODNs inhibited proliferation of BGC 823 cells.

应用相差显微镜、电子显微镜、流式细胞术等方法，观察bcl?2 ASODN 诱导BGC?823 胃癌细胞凋亡的效果。

34. The surface markers of hESCs and NSCs were detected by morphological observation, immunocytochemistry assay, flow cytometry and RT-PCR.

形态学观察结合免疫荧光细胞化学、流式细胞术和RT-PCR检测胚胎干细胞标志和神经干细胞标志;

35. One measure of malignancy is aneuploidy by flow cytometry.

恶性肿瘤的检测方法之一是通过流式细胞计显示异倍性。

36. Additionally, K562 cells were dyed by Annexin VFITC and PI for apoptosis determination by using flow cytometry.

我们以Annexin V~(FITC)标记及碘化丙啶(PI)染色后用流式细胞仪检测该浓度HHT对K562细胞凋亡的影响。

37. Antigen expression in normal organs and tumor metastases was evaluated by Western blot analysis and flow cytometry.

我们开发并描述了一个临床前的模型，用来评估免疫介导转移性乳腺癌疗法。

38. The T-cell subgroups in all patients were determined by flow cytometry before and after treatment.

所有患者在治疗前后均采用流式细胞仪检测T淋巴细胞亚群。

39. Objective: To investigate the value of multiple parameter flow cytometry in diagnosis of Non-Hodgkin's lymphoma (NHL).

摘要目的：探讨多参数流式细胞术在非霍奇金淋巴瘤(NHL)中的诊断价值。

40. Objective: To investigate the clinical value of DNA assay in patients with endometrial disease by flow cytometry (FCM).

摘要目的：探讨流式细胞术在子宫内膜疾病DNA分析中的临床应用价值。

41. Apoptosis was determined by acridine orange(AO) staining,Annexin-V/PI double staining, laser scanning cytometry(LSC) and flow cytometry (FCM).

方法 1.通过MTT比色法检测NSAIDs对细胞生长活力的影响。 2 .应用丫啶橙染色、Annexin V/PI双染色、共聚焦显微镜、流式细胞术检测细胞凋亡。

42. Methods: The expression of Fas gene in 6 strains of ovarian carcinoma cells was detected with flow cytometry and RT PCR methods.

方法 :经流式细胞术、RT PCR方法检测 6种卵巢癌细胞Fas的表达水平。

43. The Apo2.7 had been determinated with flow cytometry in 22 examples of normal trabecula tissues and 27 examples of trabecula tissue of ACG.

方法 :采用apo 2 .7分子探针 ,使用流式细胞仪检测 2 2例正常人及 2 7例闭角型青光眼小梁细胞凋亡发生率。

44. Methods:Blast cells and antigen expression relating to leukemia were identified by flow cytometry CD 45 /SSC gating and the results were compared to FSC/SSC gating procedure.

方法 :采用CD45/ SSC设门的方法确定原始细胞群 ,与异硫氰酸荧光素和藻红标记的系相关抗体联合进行三色白血病免疫表型分析 ,并与 FSC/ SSC设门方法进行比较。

45. Method: The effect of Aidi and four chemotherapy drugs on ovarian carcinoma A2780 c ells was detected by MTT assay and flow cytometry.

方法 :采用MTT法测定艾迪注射液与 4种化疗药物单独及联用对A2 780细胞的增殖抑制作用 ,运用流式细胞仪测定艾迪注射液对A2 780细胞周期的影响及凋亡率。

46. Methods:Immunofluorescent three color flow cytometry was used to study the expression of CD69 and HLA DR on T lymphocytes in 30 patients with CA and 31 normal controls.

方法 :采用免疫荧光三标记流式细胞术检测 30例CA患者外周血T细胞CD6 9和HLA DR抗原的表达 ,并以 31例正常人作为对照。

47. Methods With the use of immunohistochemistry and flow cytometry , the features of p16, p21 and DNA expression in 68 tumors and their contiguous acini were studied.

方法 应用免疫组织化学、流式细胞术对 68例腮腺肿瘤及瘤旁腺泡p16、p2 1的表达和DNA定量进行了分析。

48. Methods Morphological study,DNA gel electrophoresis,flow cytometry and fluorescein labelled annexin v were used to detect apoptosis,RT PCR to detect the apoptosis related gene.

方法 应用形态学、流式细胞术、DNA凝胶电泳、AnnexinV标记等方法检测细胞凋亡的发生 ,应用RT PCR检测凋亡相关基因Fas、bcl 2、c myc表达的变化。

49. Methods Morphological study, DNA gel electrophoresis, flow cytometry and fluorescin labeled Annexin V to detect apoptosis, RT PCR to detect the gene related with apoptosis.

方法 应用形态学方法 ,流式细胞术 (FACS) ,DNA凝胶电泳等方法检测细胞凋亡的发生 ,应用RT PCR检测凋亡相关基因Fas与FasL的变化。

50. Method Androgen receptor in 112 hypertensive men and 120 normotensive men was measured by means of flow cytometry.

方法 运用流式细胞术检测112例男性高血压患者(高血压组)及120例健康男性体检者(对照组)雄激素受体的状态。

51. Methods The samples from clinical breast cancer were harvested and the expression of Cyclins (D1, E, A, B1) was detected by using Cyclins/DNA bivariate flow cytometry.

方法 采用Cyclin/DNA双参数流式细胞术对临床上获取的乳腺癌细胞的Cyclin (CyclinD1、E、A和B1)和DNA表达进行检测。

52. Methods Ars was used to induce ECs apoptosis in vitro. ECs apoptosis, ICAM-1 expression and oxidative state and their changes after NAC treatment were detected by flow cytometry.

方法 采用亚砷酸钠(Ars)在体外诱导ECs凋亡 ,应用流式细胞术测定凋亡细胞比例、细胞间黏附分子ICAM - 1表达和细胞内氧化状态水平 ,以及NAC处理后上述指标的改变。

53. Methods Expression of LEA and CEA in colorectal carcinoma cells was detected with immunocytochemistry and flow cytometry .

方法 采用流式细胞仪和免疫细胞化学染色检测大肠癌细胞系LEA和CEA的表达。

54. Methods By using flow cytometry,we studied the expression of CD 44 and CD 54 of lymphocytes from psoriatic patients before and after UVR therapy.

方法 采用流式细胞仪对UVR治疗前后银屑病患者淋巴细胞CD4 4及CD54 的表达进行研究。

55. Methods Use flow cytometry (FCM) and immunologic techniques to detect DNA Index(DI) and cellular proliferation activity(PI).

方法 采用流式细胞术 (FCM)和免疫荧光技术对胃癌及癌前病变的细胞中DNA含量 (DI值 )、细胞增殖活性 (PI值 )进行定量检测。

56. Methods The apoptosis and expression of Bcl-2 gene of PMN in patients with UC were determined with flow cytometry.

方法 采用流式细胞术检测 18例UC患者周围血PMN凋亡及Bcl 2基因的表达。

57. Methods The techniques of cell culture, cell counts, flow cytometry, immunoprecipitation, immunohistochemistry and image analysis were employed in this study.

方法 采用细胞培养、细胞计数、流式细胞仪检测、免疫沉淀、免疫组化及图像分析等技术方法。

58. Method:Anti|tumor in a rat model and flow cytometry(FCM).

方法:体内抑瘤实验、流式细胞仪检测及电镜观察。

59. Methods: The proportion of peripheral blood CD4+CD25+ Tr was determined by flow cytometry in 31 patients with BD and 27 healthy controls.

方法:利用流式细胞仪和单克隆荧光抗体技术测定31例BD患者和27例健康对照者外周血中CD4+CD25+调节性T细胞在CD3+CD4+细胞中的比例。

60. Methods:T-lymphocytes subpopulations of the peripheral Mood ire 60 patients with urticaria and 40 healthy controls were examined by flaw cytometry.

方法:对60例荨麻疹患者用流式细胞仪检测外周血T淋巴细胞及其亚群,并与40例健康志愿者作对照。

61. Methods: DNA content and cell cycle of the cells in 18 cases of oral SCC were analized by image cytometry (ICM).

方法:应用图象细胞计(ICM)分析18例口腔鳞状细胞癌的DNA含量和细胞周期。

62. METHODS: Expression of tissue factor in tumor cells (HT1080) was analyzed by flow cytometry and immunofluorescence staining.

方法:应用流式细胞仪和免疫荧光染色法检测组织因子在纤维肉瘤细胞(HT1080)的表达情况;

63. METHODS: The expression rate of membrane Fas, FasL and that of intracellular activated caspase-3 of T lymphocyte subset were determined by flow cytometry.

方法:应用流式细胞术测定T细胞亚群表面Fas、FasL的表达率及细胞质中活化caspase3的表达率。

64. Methods:Fresh anticoagulant blood was labelled by CD14 monoclonal antibody,then monocytes were identified and the expressions of CD14 were tested by flow cytometry.

方法:新鲜抗凝全血,经CD14单克隆抗体标记,采用流式细胞仪区分单核细胞及其表面CD14的表达。

65. Methods:An immunosuppressive animal model was established by cyclophosphamide(CY).The T lymphocyte subsets were determined by flow cytometry(FCAS methods).

方法:环磷酰胺腹腔注射建立小鼠免疫功能低下模型,应用流式细胞仪对小鼠T细胞亚群进行检测。

66. Methods T-cell subpopulation in peripheral blood of 32 patients of BCC were tested with flow cytometry.

方法:用流式细胞仪技术检测了 32 例基底细胞癌患者外周血的 T 细胞亚群。

67. Methods: To measure the rate of neuronal apoptosis and expression of HSP70 by flow cytometry (FCM) and westem blots respectiovely.

方法:用流式细胞术及免疫印迹法分别检测红藻氨酸诱导癫痫发作鼠脑海马结构组织细胞不同时间的凋亡率及HSP70表达量。

68. Methods: Fas and FasL expressions of hepatoma cell line HepG2.2.15 were examined by flow cytometry.

方法:用流式细胞术检测肝癌细胞系HepG2.2.15细胞表面Fas及FasL的表达;

69. Methods: We examined the bcl-2 family expression in EoL-1 cells cultured with dbcAMP by Western blot and flow cytometry.

方法:通过免疫印记和流式细胞仪调查bcl-2家族蛋白在dbcAMP刺激的EoL-1细胞中的表达。

70. Methods: Cord blood and peripheral blood cells were analyzed by auto blood cytometry.

方法:采用全自动血细胞仪对脐静脉血和成人外周血进行分析。

71. Methods:T-lymphocytes and subsets in deciduas of 20 cases with RSA and 16 normal early pregnant women were detected by flow cytometry.

方法:采用流式细胞仪与免疫荧光技术相结合,对20例RSA患者和16例正常早孕妇女蜕膜中T淋巴细胞及其亚群进行检测。

72. Methods:The expression of CXCR4? CCR5 on NK cells of Chinese HIV/AIDS patients were analyzed with flow cytometry.

方法:采用流式细胞仪分析HIV/AIDS患者外周血NK细胞表面趋化因子受体CCR5和CXCR4的表达。

73. Methods T lymphocyte subsets were determined by flow cytometry through monoclonal antibody (MA) direct immunofluorescence technic.

方法T淋巴细胞亚群测定采用单克隆抗体直接免疫荧光技术，通过流式细胞仪测定。

74. Method The levels of CD4 + CCR5 + CCR3 - and CD4 + CCR5 - CCR3 + were measured by three-color cytometry.

方法以趋化因子受体CCR5和CCR3为Th细胞亚群表面标记,利用三色流式细胞分析法对SLE患者外周血单个核细胞(PBMCs)中CD4+T细胞亚群平衡变化进行分析。

75. Methods The levels of CD62P expression were detected with flow cytometry(FCM)in the blood of 35 patients with ATCI,30 patients with ACCE,and 33 healthy controls.

方法利用流式细胞仪测定35例ATCI患者、30例ACCE患者和33例正常对照组血液样本CD62P的表达水平。

76. METHODS The DNA quantity and SPF value were examined by flow cytometry (FCM) in 42 specimens of nasopharyngeal carcinoma and 20 cases of adjacent tissues.

方法对42例垦咽癌组织、20例相应癌旁组织,采用流式细胞术(flow cytometry,FCM)检测细胞核DNA含量和SPF值。

77. Methods Patients were divided into different groups to determine the T lymphocyte and NK cell by the FCM (Flow Cytometry).

方法将不同的结核病人分组,用流式细胞仪测定其T淋巴细胞及NK细胞。

78. Methods Cell morphology,MTT assay and flow cytometry were used to examine in leukemia K562 cell cultured in low concentration serum and free serum.

方法应用形态学、MTT分析法、流式细胞术对在低血清以及无血清条件下的白血病细胞进行检测和观察。

79. Methods The positive expression rates of CD62P and CD63 in KD patients and normal controls were measured by flow cytometry.

方法应用流式细胞技术检测32例KD患儿和15例正常对照儿的血小板活化标志物CD62P、CD63阳性表达率,同时检测BPC值。

80. Methods Percentage of apoptotic sperm(PAS) in smoking and non-smoking infertile men were tested by flow cytometry(FCM).

方法应用流式细胞术检测吸烟与不吸烟男性不育患者精子凋亡情况。