cytotoxicity

cytotoxicity

1. The AGM cytotoxicity: The HEK293 cells were cultured in medium contained 1.25%~10%(v/v) AGM for 48h, then the OD value of each group was measured by MTT assay. The statistical compare was analyzed by t-test.

AGM细胞毒性的检测：用含1.25%－10%（v/v）AGM的培养液培养HEK293细胞，培养48h后，用MTT法检测各组的吸光度，用t检验进行比较；

2. CTL cytotoxicity against B16 tumor cells was 78.5%,and the cytotoxicity against H22 tumor cells was 21.4% in B16 HSP70-peptide complex group,indicating that CTL cytotoxicity original from HIFU-treated tumor had specific anti-tumor immunity.

B16肿瘤HSP70-肽复合物组CTL对B16肿瘤细胞的杀伤率为78.5%,对H22细胞的杀伤率为21.4%,表明CTL对肿瘤细胞的杀伤作用具有特异性。

3. Tar showed no cytotoxicity to granulocytes by Evans blue exclusion.

Evans蓝排斥试验表明,烟油没有显著的细胞毒性,说明上述抑制现象是粒细胞氧合作用活性,免疫功能受到烟油抑制所致。

4. The results showed enhancement of the cytotoxicity of DNR,VP 16 by GM CSF or IL 3. The results suggest significant enhancement of the H cytotoxicity for the AML samples by GM CSF.

GM?CSF、IL?3均能增加VP?16、DNR对白血病细胞的杀伤作用；

5. All LEM-GM-CSF, LEM and DHAQ had cytotoxicity on HL60/ADM, their cytotoxic power in decrement sequence: LEM-GM-CSF, LEM, DHAQ.

LEM-GM-CSF、LEM及DHAQ对HL60/ADM细胞具有杀伤作用,且LEM-GM-CSF的杀伤作用显著强于LEM和DHAQ。

6. The depletion of MGMT by O6-benzylguanine(O6-BG) significantly increased the cytotoxicity of TMZ in resitant cell lines.

MGMT抑制剂O6-BG可明显增加TMZ对耐药系的细胞毒作用。

7. MMC has cytotoxicity effect on cultured rat C6 glioma cells in vitro,and can inhibit proliferation and induce apoptosis.

MMC能够杀伤大鼠C6胶质瘤细胞,抑制增殖,诱导凋亡,具有应用于胶质瘤治疗的潜在价值。

8. MTT assay was effective in detecting cytotoxicity activity.These data make a base for new medicine research.

MTT比色法是一种可用的体外细胞毒作用检测法,为新药品的开发提供了实验依据。

9. Cytotoxicity of FAC on HL-60 and K562 cell line was confirmed by MTT and SRB assay in vitro.

MTT法、SRB法观察FAC对体外培养的人白血病细胞HL-60、K562细胞的增殖抑制作用。

10. The cytotoxicity of NO is mainly mediated by peroxynitrite anion (ONOO-).

NO的细胞毒效应主要由过氧亚硝基阴离子(ONOO-)介导。

11. ONOO-, as a kind of strong oxidant, has very strong cytotoxicity [9]: 1.It can start the lipid overoxidation reaction. 2.

ONOO-作为一种强氧化剂，具有极强的细胞毒性[9]：1.启动脂质过氧化反应；

12. PHA can increase expression of Tac Ag on mononuclear cells from spleen of cancer patient. PHA combined with rIL-2 can increase proliferation of LAK cells but the cytotoxicity activity of the LAK cells can be reduced.

PHA可诱导肿瘤患者脾来源的单个核细胞Tac抗原的表达增强,PHA联合rIL_2应用可使LAK增殖力增强,但其杀伤力却减低,因而PHA不能用于LAK细胞诱导。

13. After Inhibiting inducible NF-KB activity by pyrrolidine dithioearbamate(PDTC), the cytotoxicity of ADM on SGC7901/ADM stomach cancer cell line was increased significantly (p<0.01).

PTDC抑制核因子一虑活化后可提高阿霉素对SGC7901/A DM胃癌细胞株的细胞毒作用(尸<0.01)。

14. Immune sera from rAAV-2-Ag85A immunized Balb/c mice were tested for anti-Ag85A antibodies by the ELISA method. Cytotoxicity of the T-lymphocyte (CTL) was determined using the ~(51)Cr release assay.

rAAV-2-Ag85A免疫Balb/c小鼠,ELISA法检测血清中抗-Ag85A抗体,~(51)Cr释放分析检测细胞毒性T淋巴细胞(CTL)活性。

15. The sulfur content of S-RCP was 8.27% and the average degree of substitution D s was 0.569. Cytotoxicity and antiviral activity of both RCP and S-RCP were studied in Vero-cell culture system.

w(S) =8.2 7% ,取代度Ds=0 .5 6 9.体外实验研究了RCP和S RCP对非洲绿猴肾细胞 (Verocellline)的细胞毒性和抗单纯疱疹病毒 2型 (HSV 2 )的活性 .

16. Tian KL,Li DH,Liu LM,et al.Study on the cytotoxicity ofchitosan and it's water souble derivatives [ J ].J Mod Clin Med Bioeng,2002,8(6):422-3.

[4]田昆仑,李东红,刘良明,等壳聚糖及其水溶性衍生物的细胞毒性研究[J]现代临床医学生物工程学杂志,2002,8(6):422-3.

17. Methods The keratinocytes of rat were seeded with the BSA-CGG,CGG,CG respectively, the cytotoxicity was detected by MTT.

[方法]应用MTT比色法,测定BSA-CGG,CGG,CG三种材料对大鼠原代表皮细胞的细胞毒性和细胞增殖率;

18. Some compounds exhibit cytotoxicity toward KB cells and P388 murine leukemia cells,and inhibitory activity against P-glycoprotein over expressing of adriamycin resistant cell line,K562/Adr cells.

一些成分在抑制KB细胞、P388白血病细胞和P-糖蛋白方面显示了较好的活性。

19. Comparison of cytotoxicity mechanism: perhaps umbilical cord blood CD3AK cells kill tumor cells to necrosis mainly, and peripheral blood CD3AK cells induced tumor cells to apoptosis mainly.

不同来源的CD3AK细胞对肿瘤细胞株细胞杀伤机制的比较:CB来源的CD3AK细胞可能主要以直接杀伤肿瘤细胞株细胞从而使其发生坏死的方式为主;

20. Compared with LAK cells, the cytotoxicity in vitro by MTT assays and the anti-tumor effects on S180 bering mice in vivo of CDC cells were tested resecively.

与LAK细胞作对比,分别用MTT法测定其体外细胞毒活性及对S180荷瘤鼠的体内抗肿瘤作用。

21. For clinic application of bone tissue engineering, reducing cytotoxicity is the most important requirement, and formability can be improved through other ways.

从骨组织工程的临床应用来看，低的细胞毒性是需要首先得到保证的，成形性能则通过其他方式来改善。

22. In ivtro cytotoxicity test indicated that the cell toxicity of the CCK leaching solution was approximately at 0 level.

体外细胞毒性实验示浸提液细胞毒性接近于0级。

23. Endpoints used in these tests usually involve general cytotoxicity, morphological changes associated with neurite outgrowth and parameters of specific neurotoxicity.

体外评价的终点包括一般细胞毒性指标、神经轴突生长的形态学指标和反映特异神经毒性的指标。

24. Ultrasound mediated microbubble destruction at 0.8 MHz , 1.0 W/cm 2, 10% duty cycle, 60 s can get the most stable effective expression of EGFP gene in Cos 7 cell and no cytotoxicity.

体外试验发现 0 .8MHz、1.0W /cm2 、10%占空比 (dutycycle)、60s超声介导 10%白蛋白微泡破裂可以有效稳定地转化EGFP基因在Cos 7细胞表达 ,且对细胞无毒副作用。

25. As a family of natural compounds,carbohydrate-based cationic polymer vectors are widely studied and applied to gene delivery due to their good biocompatibility and low cytotoxicity.

作为一类天然化合物,基于碳水化合物的阳离子聚合物载体由于其良好的生物相容性和低毒性被广泛地研究并应用于基因释放。

26. EB inhibited influenza irus-induced cell death and replication in cell culture, the authors report, without inducing significant cytotoxicity in the cultured cells.

作者报道说，EB在细胞培养中抑制流感病毒诱导的细胞凋亡和复制，而且没有显著的细胞毒性。

27. Optics and optical instruments - Contact lenses - Determination of cytotoxicity of contact lens material - Part 1 : agar overlay test and growth inhibition test.

光学和光学仪器.接触镜片.接触镜片材料的致癌性的测定.第1部分:琼酯用作细菌固体培养基中的支持物的免疫扩

28. Optics and optical instruments - Contact lenses - Determination of cytotoxicity of contact lens material - Part 1: agar overlay test and growth inhibition test.

光学和光学仪器。接触镜片。接触镜片材料的致癌性的测定。

29. The possible mechanisms may be inhibition of overproduction of NO, and reduction the cytotoxicity as free radicals of NO.

其作用机制可能与抑制NO的过量产生，减轻其作为自由基的细胞毒性有关。

30. Cytotoxicity of bone cement, nervous reflex, fat embolism and alteration of intravertebral pressure may be responsible for these changes.

其原因可能与骨水泥的毒性、神经反射、脂肪栓塞以及椎体内压力变化有关。

31. Cell growth rate test and hemolysis test were conducted to investigate the cytotoxicity and hemolysis reaction for skin reproducing membrane.

其方法是采用细胞增殖度试验和溶血试验,对医用丝素蛋白皮肤再生膜进行细胞毒性和溶血反应的实验研究。

32. To examine the dose/time-effect relationship of cytotoxicity of cadmium to human embryo nephridium cell (293 cell) and observe the effect of co-exposure to cadmium and zinc.

分析镉对肾细胞毒作用的剂量-效应关系和时间-效应关系,并观察锌对镉所致损伤的保护作用。[方法]实验采用人胚肾细胞(293细胞),设对照组、锌组、镉组、镉+锌组。

33. To evaluate the cytotoxicity of poly-lactic-acid internal fixation composing bone powder before clinical application.

分析骨粉/聚乳酸内固定材料的细胞毒性,为临床应用做前期准备。

34. Shangguan G,Xing F,Qu X.DNA binding specificity and cytotoxicity of novel antitumor agent Ge 132 derivatives,Bioorganic &Medicinal Chemistry Letters,2005,15(12):2962.

刘国湘,胡文祥.有机锗抗癌药物研究进展.中国药物化学杂志,1993,3:216.

35. Preliminary cytotoxicity showed that they possessed the same cytotoxicity as parent drug.This result may relate to the self-activation of this kind of ester in aqueous solution.

初步的体外抗肿瘤实验结果表明,取代喜树碱的酯衍生物与其母体化合物具有相近的细胞毒,这个结果可能和该类酯在溶液中自活化作用有关。

36. Ekstrand B A,Hensten-Pettersen S,Kullman A.Denture adhesives:cytotoxicity,microbial contamination,and formaldehyde content[J].J Prosthet Dent,1993,69:314.

口腔材料生物学评价:口腔材料生物性能评价导则.

37. Therefore many medical devices tests can be conducted in SHMT, such as, sterilization, sensitization, irritation, cytotoxicity, protect solution anticorrosion, etc.

可进行医疗器械产品的无菌、致敏、皮肤刺激、细胞毒性等生物学评价试验。

38. The Sr-containing CPCs possess a low cytotoxicity of 0 or1 grade, and is found to be safe to human tissue.

含锶CPC的毒性为1或0级，具有良好的生物安全性。

39. Clonogenic cell survival assay in P_(388) and HEP-3 cells treated by AMSA alone or in combination with VER revealed similar enhancement effect of VER on AMSA cytotoxicity.

在 P_(388)及 HEP-3细胞中双层琼脂或普通平皿法测定的克隆形成细胞活存率实验也证实了戊脉安有相同的增效作用。

40. In the present study,we determined EMT expression in 23 human tumor cell lines in order to verify if EMT expression correlate to SarCNU cytotoxicity.

在本研究中，我们检测EMT 在23 个人类肿瘤细胞系里的表达水平，以证实EMT 的表达是否与SarCNU 的细胞毒性有关。

41. CSO and CSO-NPs have low cytotoxicity.

壳寡糖及其纳米粒的细胞毒性较低。

42. Expression of the Fas on the lymphocytes in the women with RSA and correlation between Fas expression and NK cytotoxicity.

复发性自然流产患者外周血淋巴细胞Fas表达及其与NK细胞毒性的相关性研究

43. DNR at more than 5 ng/ml had both cytotoxicity and induction of differentiation.

大于5 ng/ml DNR有明显细胞毒效应,亦可促使分化细胞百分率增高,但分化细胞绝对数无明显增加。

44. Atmospheric fine particles can be caused the human cancer, cardiac disease,as well as can create a variety of hazardous effects including acute cytotoxicity and immunotoxicity.

大气细颗粒物诱发人体癌症、心脏病,以及引起强烈的细胞毒性和遗传毒性。

45. Trichosanthin (TCS) is a kind of anti-tumor medicine, showing great cytotoxicity to cells (JAR).

天花粉蛋白是一种抗肿瘤药物，对绒毛膜上皮癌细胞的杀伤力特别强。

46. The results show that the cytotoxicity of Ag+-pyrolytic carbon is ranked at grade 0 or 1.

实验结果表明注银热解碳的细胞毒性为0、1级，基本上无毒性；

47. After correlation analysis, the cytotoxicity showed a close correlation with the adhesiveness, phagocytic index and the activity of lysosomes, and the correlation coefficients were 0.54, 0.80, 0.67 respectively(P<0.05).

对各指标间进行相关分析发现单核细胞的细胞毒性与黏附性、吞噬指数、溶酶体活性存在紧密正相关关系,分别为0.54、0.80、0.67(P<0.05)。

48. The more potent cytotoxicity to C - 26 cells could be detected.The phagocytic activity, the secretion of IL-1, TNF, NO of the neutrophils were significantly enhanced.

对大剂量化疗后的结肠癌小鼠,仍能明显提高中性粒细胞数量、吞噬功能、杀伤C-26结肠癌细胞的活性以及分泌IL-1、TNF、NO水平。

49. This study mainly deals with cell transfection and cytotoxicity for PEI(10kD)-PBLG, a novel cationic copolymer, to observe its potential as a gene carrier.

对新型阳离子聚合物PEI(10kD)-PBLG进行研究,重点考察其基因转染效率与细胞毒性,探讨其作为基因载体的可能性。

50. Target compounds 4 and 5 were tested in a tubulin assembly assay,and in an in vitro cytotoxicity assay(KB cell line).

将目标化合物进行了抗肿瘤体外试验和微管聚合试验。

51. FCM was employed to compare the effect of IM and ATO at a concentration without cytotoxicity on the inducing apoptosis of K562/MDR1,so as to analyze the pharmacology effect of IM on ATO.

将非细胞毒作用浓度的ATO与IM联合应用于K562/MDR1,计算此时IM的IC50以及ATO应用后的逆转倍数。

52. In conclusion, Immunocal seemed to enhance the cytotoxicity of baicalein by inducing more apoptosis;this increase in apoptotic cells may be associated with the depletion of GSH in HepG2 cells.

小结：怡妙康能增强黄岑黄素的细胞毒性效果，并促进细胞凋亡，肿瘤细胞的凋亡与细胞内谷胱甘肽降低有关。

53. Heparin length in hexasaccharide is the least size for CTXA3 binding, while the length up to dp12-14 is functional important as investigated by its inhibition ability on CTX cytotoxicity.

就与心脏毒蛋白A3结合的长度来看，肝素六醣是所需要的最小结合长度；而就抑制心脏毒蛋白A3细胞毒性的性质来看，肝素链长度则需要到十二或十四醣才能发挥作用。

54. Studies have confirmed that thrombin had dual effects.Low dose of thrombin has neuroprotective effect, while high dose of thrombin has cytotoxicity.

已有研究证实,凝血酶具有双向作用,小剂量可产生神经保护作用,大剂量则具有细胞毒性作用。

55. The cytotoxicity and tubulin polymerization activity of paclitaxel’s analogues were tested.

并通过计算机模拟了类似物和微管蛋白的具体结合方式。

56. Detecting the cytotoxicity of Herceptin combined with or not with CIK cells,different effector cells,and dfferent components in the culture systems in vitro with MTT respectively.

应用MTT法检测Herceptin单独或联合CIK细胞、不同效应细胞(CIK细胞+LAK细胞)以及培养体系中不同组分的体外细胞毒性。

57. Cytotoxicity of poly-lactic-acid internal fixation composing bone powder was examined by MTT colorimetry and cell morphology test in vitro.

应用四唑盐比色法检测骨粉/聚乳酸内固定材料浸提液的细胞毒性,光学显微镜观察细胞在浸提液中的形态变化;

58. VER possessed selective enhancement of the cytotoxicity of AMSA,with the most significant enhancement in G_2M and S cells and the least enhancement in G_1 cells.

戊脉安对安吖啶细胞毒的增效作用也存在明显的时相特异性,其中对 G_2M 及 S 细胞的增效幅度最大,对 G_1细胞的增效作用较弱。 本文对其可能的临床意义进行了探讨。

59. The anti-NKG2D pAb could obviously inhibit the cytotoxicity of NK cells towards K562 and HepG2 cells and decrease their cytotoxicity to both target cells for 6.7 and 4.1 times, respectively.

抗NKG2DpAb能显著抑制NK细胞对K5 6 2、HepG2细胞的细胞毒效应 ,使NK细胞对两种靶细胞的细胞毒效应分别下降了 6 .7和 4 .1倍。

60. Antibiotic C1027, a macromolecular peptide with highly potent cytotoxicity to cultured cancer cells, was conjugated to monoclonal antibody 3A_5 and its Fab fragment separately using SPDP as the linker agent.

抗人肝癌单克隆抗体(单抗)3A_5用木瓜蛋白酶消化得到Fab片段。

61. The main cytotoxicity and mechanisms of fumonisin were also discussed.

探讨了伏马菌素主要的细胞毒性作用及机制。

62. Abstract： Objective To study the cytotoxicity of Terephthalic acid(TPA).

摘 要： 目的探讨对苯二甲酸的细胞毒性。

63. Abstract Objective:To study the correlation between cytotoxicity of daunorubicine (DNR) and harringtonine (H) to leukemic cells in vitro from 39 patients with acute myeloblastic leukemia (AML).

摘要 目的：化疗药物对急性髓性白血病细胞体外毒性进行相关性研究。

64. Abstract: Based on the previous research of bactericidal property, biocompatibility of Ag+ -implanted pyrolytic carbon was studied by cell cytotoxicity, hemolysis and platelets adhesion test.

摘要: 在注银热解碳具有明显抗菌性研究基础上，本文通过细胞毒性、溶血和血小板黏附试验综合评价了注银热解碳的生物相容性。

65. This study was aimed to investigate the transfection efficiency of adenoviral vector AD5/F35 to hematopoietic malignant cells lines of various origins and AD5/F35 cytotoxicity.

摘要本研究比较AD5/F35腺病毒载体对不同来源的血液系统恶性细胞系感染效率的差异和细胞毒性反应。

66. Object The evaluation for the cytotoxicity of nanoparticulate siliver antibacterial gel, its aim is to establish the evaluation method in vitro for the medical nanomaterial.

摘要目的对纳米银抗菌凝胶的体外细胞毒性进行评价，为建立医用纳米材料生物评价体系提供依据。

67. Objective To study the effects of native chrysotile and compounds-pretreated chrysotile on the cytotoxicity and ultrastructure in human embryo lung (HEL) cells.

摘要目的研究温石棉及用柠檬酸铝、混合稀土或亚硒酸钠预处理的温石棉对人胚肺(HEL)细胞存活率及超微结构的影响。

68. Objective: To investigate the abilitiy of HPV16E749-57 and the peptide modified from E749-57 to induce the generation of specific cytotoxicity T lymphocyte in vivo, and its therapeutical effect.

摘要目的：探讨人乳头瘤病毒16型E7抗原表位E749-57及其修饰肽是否能够在体诱导特异性细胞毒性T细胞杀伤效应，以及是否具有相应的治疗作用。

69. AIM: To investigate the effect of monoclonal antibody (McAb) AU(subscript 14-1) mediated cytotoxicity against cervical cancer U14 cell in vitro.

摘要目的：研究单克隆抗体AU(下标14-1)介导的细胞毒作用对小鼠宫颈癌(U14)细胞的杀伤效果。

70. Aim: To observe the cytotoxicity of FC, CP, calcium hydroxide, calcium hydroxide points and traditional Chinese medicine Jieeryin in vitro.

摘要目的：观察根管消毒药物甲醛甲酚(FC)、樟脑酚(CP)、氢氧化钙饱和液、氢氧化钙药尖及中药制剂洁尔阴的细胞毒性及毒性的可逆性。

71. Adenovirus vectors have the characteristics of high transfection efficiency and low cytotoxicity, and they have become most widely used vectors for gene therapy.

摘要腺病毒载体具有高效转染和低细胞毒性的特点，已成为基因治疗研究中应用最广泛的载体之一。

72. In contrast,to increase the cellular glutathione level,the cytotoxicity of PYM can be inhibited.

改变细胞内谷胱甘肽的含量可以逆转平阳霉素的毒性作用。

73. Methods CD34 + stem cells were obtained from cord blood by MACS method, CD3, CD16 and CD56 molecules expressed on cell surface were detected by Flow Cytometer. MTT method was used to detect the cytotoxicity of NK cells.

方法 采用MACS法分离脐血中CD34 +细胞 ,分别用IL 15、SCF(造血干细胞因子 )和IL 15 +SCF体外培养 ,流式细胞仪测定CD3、CD16、CD5 6分子 ,MTT法检测NK细胞杀伤活性。

74. Methods The effect of cyclosporin A on the modification of daunorubicin (DNR) accumulation, cytotoxicity, distribution in the resistant K562/ADR was examined by RT-PCR, confocal scanning laser microscopy(CSLM), fluorometry and MTT methods.

方法 采用RT- PCR、荧光法、MTT和共聚焦激光扫描显微镜等方法 ,研究 Cs A对柔红霉素 (DNR)在 Pgp耐药细胞株 K5 6 2 /ADR蓄积、毒性及分布的影响。

75. Methods The cytotoxicity of daunorubicin(DNR) was assayed by MTT method.

方法 采用甲基四唑蓝法 (MTT)测定柔红霉素 (DNR)的细胞毒性 ;

76. Methods: The photodynamic cytotoxicity of human ovarian cancer cell 3AO in different laser wavelength, energy and chlorophyll derivative (CPD4) concentration were studied by MTT colormetric assay.

方法:应用MTT比色分析法分析不同的激光波长、能量和叶绿素衍生物（CPD4）浓度对人卵巢癌3AO细胞的光动力杀伤效应。

77. Method:MTT method was used to analyze the effect of natural biofilm on the relative cell proliferation ratio of rat fibroblastes(L929) and cytotoxicity.

方法:采用MTT比色法,检测生物膜对小鼠成纤维细胞L929细胞相对增殖率的影响,评价其细胞毒性。

78. Methods:The acute toxicity,cytotoxicity and genotoxicity of three compounds 3-CH2NO2-4-CH3-DCK,3-CH2CN-4-CH3-DCK and 3-F-4-CH3-DCK were assayed using MTT test,Ames-fluctuation test and the up-down method.

方法:采用MTT比色法、上下法和Ames波动试验对3种DCK系列化合物3-CH2NO2-4-CH3-DCK(N-DCK),3-CH2CN-4-CH3-DCK(C-DCK),3-F-4-CH3-DCK(F-DCK)的细胞毒性、急性毒性和遗传毒性进行检测。

79. Methods The cytotoxicity of TPT on COC1/DDP was monitered using MTT assay and soft agaragar assay.

方法MTT比色法与软琼脂克隆形成测定TPT对人卵巢癌顺铂耐药细胞株COC1/DDP的杀伤效应;

80. Methods Selective isolation of algicolous fungi from algae was done,and antitumor activity corresponds to their lethiferous effect on brine shrimp and cytotoxicity against K562 cell was determined.

方法从样品中选择性分离得到真菌,并采用海虾生物致死法和人体慢性髓性白血病细胞K562为筛选模型对所得真菌的发酵产物进行抗肿瘤活性筛选。