explants
explants
1. Increasing lateral bud production with 6-benzylaminopurine and antibiotics pretreatments on establishment of aseptic culture for axillary explants in Dieffenbachia.
芐氨基嘌呤增进侧芽萌发及抗生素预处理对黛粉叶腋芽培植体无菌培养建立之影响。
2. Our data also showed that AgNO3 played an important role in controlling the browning of explants, and the concentration of 8.0mg/L was recommended.
0mg/L AgNO_3能较好地控制外植体的褐化，促进芽的分化。
3. We used cotyledon of S52 (FF) to be the explants, optimized shoot induction media and tested the PPT concentrations in rooting media.
14个欧洲温室型(FF):S02,S04,S07,S17,S43,S44,S45,S46,S47,S48,S49,S51,S55,S59。
4. The results showed that PYM could dramatically inhibit callus induction and differentiation of explants.
1平阳霉素(PYM)对小菊品种意大利红离体培养的叶片和茎段进行诱变处理。
5. AgNO_3 plays an important role in controlling the browning of cotyledon explants, and 6mg/L AgNO3 is recommended.
6mg/L AgNO3能很好地控制外植体的褐化,促进芽分化和芽伸长。
6. DCR+6-BA2.0 mg/L was the optimal combination ,on which 74% per cent of explants induced with an average rate of propagation 6, the highest rate of propagation 8. Addition of NAA and 2,4-D would inhibit the formation of adventitious buds.
DCR+6BA2.0mg/L是不定芽诱导的最佳组合,外植体不定芽诱导率达74%,平均增殖系数为6,最大增殖率为8。 NAA及2,4D的添加均不利于芽的诱导;
7. To avoid the browning and death of explants after co-culture and improve the transformation frequency, it was necessary to add PVP 500mg/L to co-culture medium.
N）在共培养基中添加 PVP 500mg/L，可防止外植体共培养后褐化死亡现象发生，促进转化频率的提高。
8. The activity of PAL was increased as explant turned black.
PAL活力随外植体褐变的增强而逐渐增加。
9. ZHENG J B,LIANG H Y,SUN K N,et al.Regeneration of explants of Chinese poplar leaves and its transformation with insectresistant gene[J].Journal of Hebei Forestry College,1996,11 (2):97-101.
[2]郑均宝,梁海永,孙克南,等.雄性毛白杨离体叶片再生及抗虫基因转化[J].河北林学院学报,1996,11(2):97-101.
10. Chen Jiqin,Han Liebao,Yang Chunqi,Li Xue.Effect of different explants on callus induction of perennial ryegrass[J].Grassland and Tur f,2005(4):42-47.
[7]陈季琴,韩烈保,杨纯奇,李雪.不同外植体对多年生黑麦草愈伤组织诱导的影响[J].草原与草坪,2005(4):42-47.
11. Calli from hypocotyl and cotyledon explants were induced earlier than others,and had a faster growth rates than others.Buds differentia-ted from calli were adventitious ones.
下胚轴诱导愈伤组织形成时，细胞不规则的无丝分裂少于子叶，故下胚轴离体培养得到的正常芽的比例高于子叶的；
12. Effects of Different Plant Growth Regulator Rations and Organic Compounds on Growth and Differentiation of the Explant of Taishanhong Punica granatum L.
不同植物生长调节物质配比及有机添加物对泰山红石榴外植体生长分化的影响。
13. The Xanthium sibiricum callus was induced on the MS culture medium attaching different kind and concentration hormone by Xanthium sibiricum blade, petiole and stem as explants.
不同组合及浓度配比的培养基对愈伤组织的诱导率存在显著差异,苍耳对2,4-D敏感,不宜用来诱导愈伤组织;
14. In Case 1, RPE cells grew from the explants, but were contaminated with other cells such as fibroblasts and melanocytes, and no pure RPE cultures were obtained by explantation.
个案一采传统组织体外培养法，结果发现虽然视网膜色素上皮细胞顺利生长，但是被纤维母细胞，黑色素细胞等其他种类细胞污染而驳杂不纯。
15. Embryogenic callus can produced directly from cotyledon, hypocotyle and radical, or from callus producing from explants.
中棉所19的体细胞胚胎发生有两种情况：一是由外植体直接诱导获得胚性愈伤组织和体细胞胚；
16. The experimental results on tissue culture of Begonia clatior showed that using the leaves as explants was best.
丽格秋海棠组织培养试验结果表明,利用丽格秋海棠叶片作外植体是较好的取材部位;
17. The addition of acetosyringone,the concentration of bacterium suspension,the time of inoculation,different explants as well as seedling age exhibited certain influences on transformation.
乙酰丁香酮的添加、农杆菌液的浓度、共培养时间、外植体取材部位及时间，均可以影响发状根的诱导频率。
18. Embryogenic callus and somatic embryos could be directly obtained on the medium only with ZT, but it was influenced by explant sources.
二是先诱导获得愈伤组织，再经继代培养获得胚胎发生。
19. Species and varieties,transformation methods. target genes transformed,explants,transformation frequency andits influencing factors involved in citrus transgenic research are reviewed,And the prospect of research is also covered.
从柑橘种及品种、转化方法、导入的目的基因、转化所用的外植体、转基因频率及其影响因素等方面对柑橘转基因的现状进行了综述,并提出了下一步的研究重点。
20. In propagation through enhanced axillary branching of two-years old Eucommia ulmoides, May proved to be a favorite time for explant collection.
以2年生杜仲带芽茎段为起始材料进行离体快繁时，最佳取材时间是5月份。
21. It is obvious that callus induction and proliferation of gerbera need growth regulators, and are influenced by varieties and explants of different parts of the plants.
以上结果说明，生长素是非洲菊愈伤组织诱导和增殖所必须的，非洲菊愈伤组织诱导、增殖和芽再生方式受品种及外植体类型的影响。
22. Floral organ culture and rapid propagation of Lilium orential "Sorbonne" were carried out using pedicle, receptacle, petal, and filament as explants.
以东方百合 索邦 L ilium orential Sorbonne 的花梗、花托、花瓣和花丝为外植体 ,进行了离体培养与快速繁殖研究 .
23. The oriental lily squamas of Sorbonne were used as explants for tissue culture.
以东方百合“索邦”的鳞片为外植体,进行组织培养技术研究。
24. Tissue culture of Dioscorea opposita "foshou" was studied with explants such as tubers,leaves,stem segments.
以佛手山药块茎、叶片、茎段为外植体,探讨了其组织培养技术。
25. Using bulblets of Freesia refracta Shangnongjinghuanghou as explants,the influence of plant hormone to its in vitro culture and plant regeneration was studied in this paper.
以小苍兰优良品种“上农金黄后”的小籽球为外植体,研究了植物生长调节剂对其球茎离体培养及植株再生的影响。
26. The shoot tips with a length of 0.5~0.8 mm is suitable for explants,which came from Chrysanthemum with CMV.Callus grew well on MS+BA2.0 mg/L+NAA0.1 mg/L and 96.0% explants formed.
以患花叶病菊花0.5~0.8 mm幼嫩茎尖为外植体,愈伤组织的诱导在MS+BA2.0mg/L+NAA0.1 mg/L培养基上效果最好,诱导率达96.0%;
27. Stems with axillary buds from Cardinal, elite of China rose, were used as explants for in vitro rapid propagation.
以月季良种红衣主教带腋芽茎段为外植体进行离体快速繁殖研究 .
28. Effective plant regeneration was obtained on MS medium supplemented with NAA 1mg/L and BA 2mg/L,using leaves of basal bulb as explants in Amaryllis vittata. The highest callus induction rate was 93.3% and shoot induction rate was 90.0%.
以朱顶红鳞茎基部鳞片为外植体 ,在MS +NAA 1mg/L +BA 2mg/L培养基上愈伤组织的诱导频率最高 ,为 93 .3% ,愈伤组织上可以直接生芽 ,生芽频率为 90 .0%。
29. Use test-tube seedlings of Chinese date Goutouzao and Junzao as explants,effect of TDZ and 6-BA on subculture of Chinese date were studied and selected its suitable composition in subculture.
以狗头枣和骏枣试管苗为材料,研究了TDZ和6-BA在枣树试管苗继代培养中的效应并筛选了适宜组合。
30. Embryonic calli could be obtained from explants such as terminal buds and axillary buds of hybrid giant napier inoculated in induced media during tissue culture.
以皇竹草腋芽或顶芽作外植体，接种在脱分化培养基上培养，可获得胚性愈伤组织。
31. The regeneration system of tissue culture of Symplocos tetagona Chen et.Y.F.Wu was established using seeds as explants.
以种子为外植体，初步建立了棱角山矾的组织培养体系。
32. The abnormal rate of chromosome number was highest inthe callus with seed embryo as explant,but the callus grewout no budor very few buds with abnormal bud shape.
以种胚为外植体的愈伤组织中染色体数目异常的频率最高,但愈伤组织不出芽或出芽量极少,且芽形态不正常。
33. Callus was induced using seeds as explant on Dioscorea nipponica Makino,the best culture media is MS+6-BA3.0 mg/L+NAA2.0 mg/L and the highest frequency is 18.9%.
以穿龙薯蓣种子为外植体诱导出了愈伤组织,最佳培养基为MS+6-BA3.0mg/L+NAA2.0mg/L,最高出愈率为18.9%;
34. Floral buds of Passiflora edulis Sims were used as explants in tissue culture.
以紫果西番莲的花蕾为材料,进行组织培养愈伤组织诱导的研究。
35. These young stems as explants placed on different media formed calli,whose amount then increased by different cultures on different media and for different generations.
以金边瑞香茎段为外植体,探讨了不同培养基对愈伤组织诱导、愈伤组织分化的影响。
36. Using the shoots as the explants, the effect of different factors on Opuntia milpa alta haw in vitro is examined.
以食用仙人掌的茎片为外植体,研究了食用仙人掌离体快繁的若干影响因素。
37. The axillary bud on young shoot of Barbados nut was used as explants,adding different concentration of plant hormone combinations(6-BA and IBA) on minimal medium(MS),to carry out buds inducing and multiplication culture.
以麻疯树幼嫩枝条腋芽为外植体,以M S为基本培养基,附加不同浓度的激素组合(6 BA与IBA)进行芽诱导和增殖培养。
38. Callus was obtained from the terminal bud explants of Ficus robusta on Murashige and Skoog(MS) basel medium supplemented with 2.0 mg L-1 BA and 0.1 mg L-1 NAA.
以黄斑橡胶榕(Ficus robusta“Yellow spot”)的顶芽为外植体进行离体培养与植株再生研究。 结果表明,黄斑橡胶榕的诱导培养基以MS+BA 2.0 mg L-1+NAA 0.1 mg L-1为宜;
39. The use of low-strength soft initiation medium (1/4MS+2% sucrose+0.5% agar,supplemented with 0.5mg/L BAP and O.05mg/L IBA) was effective in preventing browning of the explants.
低浓度营养成份的软质启动培养基（1/4MS＋2%蔗糖＋0.5%琼脂，附加0.5mg/LBAP和0.05mg/LIBA）能有效地防止外植体的褐化死亡；
40. In the rakkyo callus induction experiment, 0.5g/L PVP can effectively inhabitting the explant browning.
使用0.5g/L PVP进行薤的鳞片愈伤组织诱导时可以有效地抑制外植体的褐化。
41. After co-culture was over, the explants were directly transferred to the selection medium MSB2 (MSB1+Cef500mg/L+Km100mg/L) for three weeks.
共培养完成后，将外植体直接转入筛选培养基MSB_2(MSB_1+500mg/L Cef+100mg/L Km)中进行分化培养，每3周继代一次。
42. Explants came from winter and the old explants had low brown rate. The active carbon played a role in inhibiting browning.
冬季外植体褐变率明显低,较老的组织褐变较轻,活性炭有较好的抑制褐变的作用。
43. Factors affecting explant browning in tissue culture of Hippophae rhamnoides L.
几种影响沙棘组织培养外植体褐化因子分析。
44. After 1 week, cells grew from the edge of original 1mm3 BSM explant, and they overspread after 3-4 weeks. Both ASMC and BSMC are zonary or spindly, with smooth membrane.
分离的支气管平滑肌剪碎成1mm3的小块采用植块培养的方法较好,1 周后有细胞从边缘爬出,大约3~4 周后铺满培养皿,所得细胞呈梭形,纯度较高。
45. A section of liver explant is provided.
切片示切除的肝脏。
46. In initial culture ,it can effectively prevent the explant from browning to append 0.5g/L PVP to culture medium.
初代培养时,培养基中加入 0.5 g/L 的 PVP,可以有效地防止组织培养过程中外植体褐化。
47. Establishment and identification of a system to transform hairy roots of Echinacea purpurea. Hairy roots were induced from the wounded brim of the explants, 25 days after inoculation of Echinacea purpurea explants with A.
利用A4、R1601、R1000三种发根农杆菌诱导紫锥菊的遗传转化,建立紫锥菊毛状根诱导体系。
48. The mature embryos were cultivated on NB media with different hormones,by using the mature embryos in rice as explants,and regeneration plants were obtained successfully.
利用水稻成熟胚为外植体,接种在NB培养基上,分别附加不同的外源激素,以诱导愈伤组织并促其分化,最终获得水稻再生植株。
49. Calli induction: Explants was death in MS+6-BA+NAA medium, both leaves and stem segments were chosen, can’t be differentiated.
半枝莲愈伤的诱导：以叶片和茎段为外植体，在激素6-BA和NAA组合的培养基上诱导愈伤，均未成功。
50. E or F-plus can be more rapid start-up CHI explants of dedifferentiation.
单加E或F均能较快速的启动CHI外植体的脱分化。
51. So I can understand as soos as you explant to me.
原来如此!一经你解释我就明白了。
52. Methods Protocorm-like bodies and callus of DCWL subcultured for 30 days was used as the explants,N6 was used as the basic culture with phytohormone added,and fungal extracts as the elicitor.
取继代培养30d的铁皮石斛类原球茎作接种材料,以N6为基本培养基,加入植物激素,并以真菌提取物作诱导子,对类原球茎和愈伤组织进行诱导培养。
53. AgNO?3 played and important role in controlling the browning of explants, and 8.0 mg/L was recommended.
另外,8 0mg/LAgNO3能有效地控制外植体的褐化,促进芽的分化。
54. The adventitious bud differentiation rate of different explants types was leaf (95.1%) > stem segment (66.3%)> root segment (61.5%).
叶片、茎段和根段三种外植体类型的再生频率大小顺序依次为叶片(95.1%)>茎段(66.3%)>根段(61.5%)。
55. The time of dipping leaf pieces in suspension affected not only the gene transfer efficiency, but also the inhibition by cefotaxime of the growth of Agrobacterium on explants.
叶片在菌液中的浸泡时间不仅影响基因转移效率，而且影响头孢霉素对外植体上农杆菌生长的抑制效果。
56. Zygotic embryo is better than vegetative materials when used for explants.
合子胚作为外植体明显好于营养器官作为外植体。
57. Explanation of plates 1.Differentiation of pepper cotyledon in the differentiation medium;2.Buds of explants enlongation in the growth medium;3.Induce of roots of pepper shoots;4.Regenerated pepper plants were transplanted into normal soil.
图版说明1.辣椒子叶在分化培养基中分化;2.外植体分化的不定芽在生长培养基中伸长;3.再生小苗不定根的诱导;4.再生苗的移栽。
58. Adjacent explants were connected to each other by the migrating cells with the occurrence of autorhythmic beating of the explants.
在三维胶原中,心脏组织块生长良好,迁出的细胞将相邻组织块连接起来,组织块有自发的搏动。
59. Among cotyledon, petiole of cotyledon, hypocotyl, and radicle, hypocotyl has the most responsive explants to2,4- D.
在子叶、叶柄、胚轴以及胚根四者当中,下胚轴对2,4-D的反应状况最好。
60. A peak value of ABA and IAA content and a low value of GA3 content in explant were detected at the beginning of embryonic calli generation.
在胚性愈伤组织开始发生之际，外植体的ABA和IAA含量出现峰值，GA3含量出现低谷。
61. Kentucky bluegrass regeneration system research progress in the world were summarized in this paper on the influencing factors such as the choice of explants、culture conditions、genotype and so on.
在草地早熟禾再生体系建立的过程中,就其影响因素如外植体的选择、培养条件、基因型等方面综述了国内外草地早熟禾再生体系的研究进展。
62. The MS medium supplemented with 0.2mg/L 6-BA,0.01mg/L IAA or 0.2mg/L 6-BA,0.01mg/L IBA were found promising effect in elongation, which is a key step of in vitro propagation of castor( Ricinus communis ) from meristematic explants.
在蓖麻的茎尖培养中,幼芽伸长是关键的一步,添加0.2mg/L 6-BA,0.01mg/L IAA或0.2mg/L 6-BA,0.01mg/L IBA的MS培养基对蓖麻幼芽的伸长有较好的效果。
63. The optimal explant in tissue culture was stem section,and the frequence of callus induction can reach 50%.
在进行组织培养时可优先考虑采用其茎作为外植体进行愈伤组织诱导,其诱导率可达50%。
64. Cotyledon and cotyledon node are all good explant for cucumber transformation compared to hypocotyledonary axis.
在黄瓜转化与再生过程中，选择黄瓜子叶、子叶节作为外植体优于下胚轴。
65. The Ecs gained from the original skin explants which were cultured after 7 days and from epidermal outgrowth lost their ability to stimulate allogeneic lymphocyte transformation in MEcLR.
培养7天后的Ecs及新生Ecs在MEcLR 中失去刺激异体淋巴细胞转化的能力。
66. In five hormone combinations, the effect of the basic medium plus IAA 0,3mg/L and LBA 0.1mg/L was best for the explant to take root earlier and make the stem and leaves grow more rapidly.
培养基中激素配比以基本培养基+IAA 0.3mg/升+IBA0.1mg/升对诱导外植体早生根并促进茎叶生长的效果最显著。
67. The differences of these characteristics between graft-origin and basal-sprout explants remained in the first 3 subcultures, however, they disappeared in the late.
复壮外植体与基部萌条的差异在最初的3次继代培养中仍然存在，但在以后的继代培养中消失了。
68. The different cutting-out methods of explants affected the initial h ealing time of callus inductions, and could lead to time reduction.
外植体的不同切取方式对愈伤组织诱导的始愈时间有影响 ,经切取后可缩短始愈时间 ;
69. It is the factors affected plant regeneration in explant culture in Euphorbia pulchrrima willd to different explants or aurin or cytokinin or the combination of the auxin and cytokinin.
外植体的类型、植物生长素、细胞分裂素的类型及二者的不同组合均对一品红在离体条件下植株的再生具有影响。
70. Embryogenic callus was obtained only from hypocotyl explants of Astragalus adsurgens and light inhibited the formation of embryogenic callus.
外植体类型和光照条件决定沙打旺胚性愈伤组织的形成。
71. After dedifferentlation and redifferentiation,the explants had three fates: abnormal plantlets, death and healthy plants.
外植体通过诱导经历脱分化和再分化，最后大致有三种去处：诱导出畸形苗，死亡和诱导出正常植株。
72. It focused the discussion on the advances of selection of explants, light, temperature and 6-BA, NAA, ethylene, which affecting inflorescence induction and flowering of Composite.
如外植体的选择、光照和温度，以及6-BA、NAA、乙烯等植物生长调节物质等的研究进展作了重点评述。
73. The frequency of shoot regeneration from cotyledon explants of fifty days fast vegetable and Zhejiangzaoshu No. 5 is higher. So both varieties were used as the material for transgenic experiment.
子叶外植体的芽分化率以50天快菜和浙江早熟5号为高，故此以这两个品种作为转基因的试验材料。
74. The capabilityof neurlte regeneration of retinal explant was decreased with the passage of time after birth, and it was lost after two weeks old.
实验结果显示大白鼠的视网膜于组织培养中随著年龄的增加而逐渐递减其神经轴突的再生能力，而在出生后两星期后完全丧失此功能。
75. Studies on the micropropagation of5cultivars of almond,Bulgaria1,Nonpareil,Fritz,Prize and Neplus,was conducted with node explants.
对5个引进的美国扁桃品种Bulgaria1、Nonpareil、Fritz、Prize和Neplus的微繁殖进行了研究。
76. The explant tissue culture was compared with the typsin digestion culture.
对比了组织块培养和消化培养两种培养方法。
77. Hypocotyl,cotyledon,stem,leaf blade,leaf petiole explants of tomato were cultured on MS medium supplemented with 2 mg/L BA and 0.2 mg/L IAA for induction of calli and bud formation.
对番茄下胚轴、子叶、茎段、叶片、叶柄不同类型外植体离体培养中有关细胞启动、分裂、分化以及器官发生作了细胞组织学观察。
78. This article makes a relatively all-around brief in explant,condition of cultivate,methods,result to the headway of tissue culture research on Nervilia fordii(Hance) Schltr.
对青天葵组织培养从外植体、培养条件、方法和结果等方面做了较全面的总结,青天葵是可以通过组织培养达到快速繁殖目的的。
79. The Spathiphyllum floribundum cv.Cupid explants were inoculated in MS substratum with BA 0-4.0 mg/L .
将"Cupid"白鹤芋外植体分别接种在含BA 0-4.0 mg/L的MS培养基中。
80. Plant expression vector p35EZ was constructed to transform the explants of Cucumis sativus L. by Agrobacterium tumefaciens (Smith et Townsend) Conn Ti plasmid_mediated method.
将该基因克隆在pBI1 2 1的 35S启动子和Nos终止子之间 ,得到植物表达载体p35EZ。 通过根癌农杆菌 (Agrobacteriumtumefaciens (SmithetTownsend)Conn)介导的方法转化黄瓜 (CucumissativusL .)
