hybridoma

hybridoma

1. 1975 Hybridoma technology deeloped for production of monoclonal antibodies.

1975年,杂交瘤技术被应用于生产单克隆抗体.

2. 1975 Hybridoma technology developed for production of monoclonal antibodies.

1975年,杂交瘤技术被应用于生产单克隆抗体。

3. Estabishment and characterization of mouse hybridoma cell lines secreting monoclonal antibody against annexin B1

1单克隆抗体杂交瘤细胞株的建立和特性鉴定

4. BALB/c mice were immunized with viable hybridoma cells of 5A 8secreting specific McAb (Ab1) against human gl(?)

5A_8为抗人胶质瘤相关抗原的单克隆抗体(Ab_1)。

5. B lymphocyte hybridoma

B淋巴细胞杂交瘤

6. B cell hybridoma

B细胞杂交瘤

7. DMSO Arrested Hybridoma Cells for Enhanced Antibody Production

DMSO抑制杂交瘤增殖促进抗体的分泌

8. Studies on Dot-ELISA for Screening Hybridoma Secreting Monoclonal Antibodies Against Beet Mosaic Virus

Dot-ELISA及其在植物病毒单克隆抗体筛选中的应用

9. JZ-1, A HYBRIDOMA LINE SECRETING MONOCLONAL ANTIB ODY AGAINST HLA CLASS I ANTIGEN LIGHT CHAIN-BETA, MICROGLOBULIN

JZ-1,一株分泌抗HLAI类抗原轻链单克隆抗体的小鼠杂交瘤系

10. Culture of MG7 hybridoma cells and detection of antigen-binding affinity of MG7 mAb by ELISA

MG_7杂交瘤细胞的培养及利用ELISA检测MG_7单抗的抗原结合活性。

11. T lymphocyte hybridoma

T淋巴细胞杂交瘤

12. T cell hybridoma

T细胞杂交瘤

13. The Comparison of IL-2 and IL-6 Activity Level in a Variety of Feeder Cell Culture Supernatants and Conditioned Media for Hybridoma Technique

不同杂交瘤饲养细胞上清和条件培养液中IL-2和IL-6水平的比较

14. To develop low protein medium, the effect of various polymers on hybridoma cell growth was investigated in batch cultures with low serum levels, including PEG, PVA, PVP, Dextran etc.

为促进杂交瘤细胞在低血清条件下的生长及无血清培养基的开发，通过向培养基中添加一定初始浓度的高分子物质，在批式培养中研究了它们对杂交瘤细胞生长的影响。

15. hybrid hybridoma

二次杂交瘤

16. Keywords human mammaglobin;breast cancer;prokaryotic expression system;protein purification;hybridoma;monoclonal antibody;

人乳腺珠蛋白;乳腺癌;原核表达系统;蛋白纯化;杂交瘤技术;单克隆抗体;

17. Establishment of Human Thyrotropin Monoclonal Antibody in Hybridoma Cell Lines

人促甲状腺激素单克隆抗体杂交瘤细胞株的建立

18. THE IN VITRO INDUCTION OF ANTIBODY RESPONSES OF HUMAN LYMPHOCYTES AND ITS APPLICATION TO HYBRIDOMA PRODUCTION

人淋巴细胞的体外抗体诱发及其在杂交瘤中的应用

19. Keywords Human chorionic gonadotrophin;Monoclonal antibody;Hybridoma cell lines;

人绒毛膜促性腺激素;单克隆抗体;杂交瘤细胞株;

20. Establishment or Hybridoma Cell Strain Secreting Human Hepatocyte Growth Factor

人肝细胞生长因子杂交瘤细胞株的建立

21. Recombinant human cTnI protein was used as antigen to immunize BALB/c mice. Monoclonal anti-bodies against cTnI were prepared by normal hybridoma technology. The relative affinity of mAbs was determined by ELISA.

以基因重组的cTnI蛋白为抗原,常规方法免疫BALB/c小鼠,取其脾细胞与NS1细胞融合,获得稳定分泌cTnImAb的杂交瘤细胞株,ELISA检测mAb的相对亲和力;

22. Keywords OX40L;costimulatory signals;hybridoma;monoclonal antibody;leukemia;

共刺激信号;杂交瘤;单克隆抗体;白血病;

23. Keywords Human sodium iodide symporter Monoclonal antibody Hybridoma Western-blot Immunohistochemical staining;

关键词人钠碘转运体;单克隆抗体;杂交瘤;电泳印渍检测法;免疫组化;

24. Keywords Japanese encephalitis virus;antibody, anti-idiotypic, monoclonal;hybridoma;receptor, virus;detection;early diagnosis;

关键词日本脑炎病毒;单克隆抗体;抗独特型;杂交瘤;病毒受体;检测;早期诊断;

25. McAb hybridoma cells then were injected into other mice abdominal cavity to prepare ascites again.

再将此分离出的杂交瘤细胞，注入其他小鼠腹腔又制得腹水。

26. ESTABLISHMENT OF HYBRIDOMA CELL LINES SECRETING MONOCLONAL ANTIBODY AGAINST HUMAN RED CELLS(ANTI-A OR ANTI-B)AND PRELIMINARY APPLICATION IN BLOOD GROUPING

分泌抗人A型、B型单克隆抗体细胞系的建立及初步应用

27. Establishment of Hybridoma Cell Lines Producting Monoclonal Antibodies Against Human Alpha-Fetoprotein

分泌抗人甲胎蛋白单克隆抗体杂交瘤细胞株的建立

28. Establishment of Hybridoma Cell Lines Secreting Monoclonal Antibody Against Ampicilline

分泌抗氨苄青霉素单克隆抗体杂交瘤细胞株的建立

29. Establishment of hybridoma cell strains secreting McAbs against Yersinia Pestis F1 antigen

分泌抗鼠疫耶尔森菌F1抗原单克隆抗体杂交瘤株的建立

30. Keywords 4-1BBL;Monoclonal antibody;Costimulatory signals;Hybridoma;

单克隆抗体;共刺激信号;杂交瘤;

31. Identification of the hybridoma cell lines immunized by the antigens of Borna disease virus

博尔纳病病毒抗原免疫的杂交瘤细胞系的评价

32. Keywords Ovalbumin;Monoclone antibody;Hybridoma cell lines;

卵清蛋白;单克隆抗体;杂交瘤细胞株;

33. Keywords primary hepatocyte;hybridoma technique;hepat-hybridoma cells;cytochrome P450;in vitro drug metabolism study;

原代肝细胞;杂交瘤技术;肝杂交瘤细胞;细胞色素P450酶;体外药物代谢研究;

34. The ideal monoclonal antibody was ought to origin from human, but which could not to come true in nowdays, because of hybridoma technique、affinity and so on.

因此，应用于临床的理想抗体应是人源的，但人-人杂交瘤技术目前尚未突破，且还存在人-人杂交瘤体外传代不稳定、抗体亲合力低及产量不高等问题。

35. In 1. 5 L Celligen bioreactor, when pluronic F-68 concentration is 0. 10%(W/V) in medium and agation speed is 70r/min the hybridoma cell can grow normally.

在1.5升GelliGen生物反应器中,培养基添加0.10%Pluronic F-68、搅拌转速70r/min下细胞正常生长。

36. Growth and Metabolism of Hybridoma Cells Cultured in Flak and Spinner Bottles

在静态和动态培养条件下杂交瘤细胞的生长和代谢

37. Any of a class of antibodies produced in the laboratory by identical offspring of a hybridoma; very specific for a particular location in the body.

实验室里由杂种细胞同卵双生的后代产生的一类抗体，位于体内一个特别的位置。

38. Used the hybridoma technique to prepare the PPPA2?P5?A2?P5?A monoclonal antibodies by immunzing Balb/c mice with PPPA2?P5?A2?P5?A coupled to BSA.

将PPPA2?P5?A2?P5?A（2?－5?P3A3）与甲基牛血清白蛋白偶联后免疫Balb/c小鼠，用杂交瘤技术获得了抗2?－5?P3A3单克隆抗体。

39. Growth and Antibody Production of Resultant Hybridoma Cells in a Double-mouthed Roller

小鼠杂交瘤细胞在双口滚动培养瓶中的生长及抗体分泌

40. A hybridoma secreting monoclonal antibody (McAb) against human antidiu-retic hormone (ADH) was obtained by fusion of Sp2/0 myeloma cells with spleen cells from BALB/c mice immunized with ADH.

应用B淋巴细胞杂交瘤技术,成功地建立了分泌抗抗利尿激素(ADH)的单克隆抗体细胞株。

41. Effects of Limited Substrates on the Growth, Metabolism and Monoclonal Antibody Formation of Hybridoma Cells

底物限制对杂交瘤细胞生长、代谢和单抗生成的影响

42. Yuanxing Zhang, Wangfun Fong, Pingpei Yung (1998) Optimization in hybridoma cell culture.

张立，沈红，张元兴*(2000)在动态和静态培养条件下杂交瘤细胞的生长和代谢。

43. The chromosome number of post-fused hybridoma cell was near to the cumulative chromosome number of splenic cell and myeloma cell.

当脾细胞与凯骨髓瘤细胞融合形成杂交瘤细胞后，其染色体数目接近两亲本细胞染色体数目之和。

44. The study of fact that influence the anti-A antibody of hybridoma

影响抗A杂交瘤细胞抗体分泌的部分因素

45. Keywords APA microcapsule;hybridoma cells;monoclonal antibodies;cytomedicine;

微囊;杂交瘤细胞;单克隆抗体;细胞制剂;

46. Results of controlled experiments with hybridoma cells indicated that theretention efficiency of viable cells was higher th...

所建过程模型对过程操作具有预见性,可用于过程参数的估计,并可指导沉降装置的设备放大。

47. The hybridoma cell line with the collection number of CGMCCNO.1908 is used for a secrete anti-URG11 monoclonal antibody hybridoma cell line.

所述的保藏号为CGMCCNO.1908的杂交瘤细胞株用于分泌抗URG11单抗的杂交瘤细胞株。

48. Establishment and clinical application of the hybridoma cell bank of monoclonal antibodies against 8 types of main respiratory viruses

抗8种主要呼吸道病毒单抗杂交瘤细胞库的建立和临床应用

49. Establishment of Hybridoma Cell Secreting McAb Against DNA and Characterization of The Secreted McAb

抗DNA单克隆抗体杂交瘤细胞株的建立及其抗体的鉴定

50. Establishment of anti-HBV Terminal Protein antibody producing hybridoma cell lines and characterization of their monoclonal antibodies

抗HBV末端蛋白杂交瘤细胞系的建立及其单克隆抗体鉴定

51. Establishment of anti-HBV reverse transcriptase antibody producing hybridoma ce ll lines and characterization of their monoclonal antibodies

抗HBV逆转录酶杂交瘤细胞系的建立及其单克隆抗体鉴定

52. The preliminary determination of anti-TPO monoclonal antibody hybridoma cell line

抗TPO单克隆抗体杂交瘤细胞系的建立

53. Establishment and characterization of two hybridoma cell lines secreting anti-osteosarcoma antibody

抗人成骨肉瘤杂交瘤细胞系的建立及其特性鉴定

54. The Establishment of a Hybridoma against Human Colon Carcinoma and the Characterization of the Monoclonal Antibody

抗人结肠癌杂交瘤细胞系的建立及单克隆抗体特性研究

55. Keywords Monoclonal antibody;Hybridoma cell;Ascites;Mice;

抗体;单克隆抗体;腹水;小鼠;

56. Filtration of hybridoma lines of monoclonal antibody and development of colloidal gold-labeled strip for rapid detection of chloramphenicol

抗氯霉素单克隆抗体杂交瘤细胞株的筛选及金标试纸检测方法的建立

57. Establishment of Mouse Hybridoma Cell Lines Secreting Monoclonal Antibody Against Cysticercus Cellulosae Antigen and Characterization

抗猪囊虫单克隆抗体杂交瘤细胞株的建立和特性鉴定

58. Development of hybridoma cell lines excreting monoclonal antibodies against zearalenone

抗玉米赤霉烯酮单克隆杂交瘤细胞系的建立及单抗生产

59. Development of hybridoma cell lines excreting monoclonal antibodies against Ochratoxin A

抗赭曲霉素A单克隆杂交瘤细胞系的建立及特性

60. Establishment and Primary Identification of Hybridoma of Anti- zona Monoclonal Antibody

抗鱼卵透明带单克隆抗体淋巴细胞杂交瘤的建立及初步鉴定

61. When BALB/C mice which had rejected the anti-DNP IgE producing hybridoma B63, were im-munized with DNP proteins, they produced much less anti-DNP antibodies than control (normai) mice.

排斥杂交瘤B_(53)(分泌抗DNP IgE)的BALB/C小鼠(Hy·Rej·mice),注射DNP蛋白质抗原后,几乎不形成抗DNP抗体。

62. The cTnI was used to immunize Balb/c mice by route method. The spenocytes were fused with SP2/0 myeloma cell lines. Six hybridoma cell lines,secreting stably McAbs to cTnI antigen have been obtained and screened.

方法 :应用cTnI免疫Balb/c小鼠 ,取脾细胞与SP2 /0骨髓瘤细胞进行细胞融合筛选 ,获得分泌高效价抗cTnI抗体细胞株 6株。

63. Methods We first applied the Agu to immunize BABL/c mouse, and fused the spleen cells of the immunized mouse with SP2/0 myeloma cells. Enzymelinked immunosorbent assay (ELISA) was used to screen the McAb which the hybridoma cells produced.

方法 使用猪囊虫尿素溶性抗原 ( Ag- u)并用水溶性抗原 ( Ag- w)作对照分别免疫 BABL / c小鼠 ,取其脾细胞分别与 SP2 / 0骨髓瘤细胞融合 ,EL ISA法进行筛选。

64. METHODS: The VLand VH genes were amplified and cloned from the hybridoma 1F11 secreting anti-rh-bFGF mouse monoclonal antibody mAb by RT-PCR, The CL and CH genes were amplified and cloned from the plasmid pMDHC and pMDLC.

方法：从分泌抗rh-bFGF鼠单抗mAb 1F11的杂交瘤细胞中通过RT-PCR扩增、克隆V_L、V_H基因，从质粒pMDHC和pMDLC中扩增、克隆C_L、C_H基因。

65. by-passing hybridoma

旁路细胞杂交融合瘤

66. Keywords Trichinella spiralis;muscle larvae;ES antigen;hybridoma;McAb;

旋毛虫;肌幼虫;排泄分泌抗原;杂交瘤细胞;单克隆抗体;

67. Hybridoma growth and antibody production in protein-free medium

无蛋白培养中杂交瘤细胞的生长与单克隆抗体分泌

68. The present invention discloses a mouse anti-human CD41 monoclonal antibody hybridoma cell system, a monoclonal antibody and an immunohistochemical kit and an application thereof.

本发明公开了一种鼠抗人CD41单克隆抗体杂交瘤细胞系、单克隆抗体、及其免疫组化试剂盒和应用。

69. Keywords Leptin Hybridoma Monoclonal antibody （McAb）;

杂交瘤;单克隆抗体;

70. Keywords GL50;Hybridoma;Monoclonal antibody;Costimulatory molecular;

杂交瘤;单克隆抗体;共刺激分子;

71. Keywords hybridoma;monoclonal antibody;Neisseria gonorrhoeae;

杂交瘤;单克隆抗体;淋球菌;

72. Keywords BTLA;Hybridoma;Monoclonal antibody;Protein purification;

杂交瘤;单克隆抗体;蛋白纯化;

73. Keywords hybridoma;peptide mass fingerprinting;chimeric antibody;

杂交瘤;质谱分析;嵌合抗体;

74. Hybridoma and Myeloma Cell Culture SFM

杂交瘤和骨髓瘤细胞培养专用无血清培养基

75. hybridoma technology

杂交瘤技术学

76. With the establishment of lymphocyte hybridoma techniques,mAb develop for a long term which changed from diagnose tools to therapeutic medicine.

杂交瘤技术建立以来,单抗研究走过了漫长而又曲折的历程,分子生物学的发展使单抗从诊断工具变为有效的治疗药物。

77. hybridoma product

杂交瘤生成

78. hybridoma growth factor

杂交瘤生长因子

79. hybridoma dish

杂交瘤皿

80. hybridoma cell

杂交瘤细胞