orfe

orfe

1. Fdr3 eDNA including a 1068bp ORF was 3. 3kbin length. The results supposed that the fdr3 Mrna was elevated under irondeficiency condition by Northern hybradization in maize and wheat roots.

3kb，包括一个1068bp的开放阅读框，玉米，小麦的Northern杂交均证明缺铁加强表达（见霍春艳和宋秀芳论文）。

2. Nucleic acid sequence analysis showed it contained a 363 integrate ORF, encoding 120 amino acids. It was named gcI. And gcI is located at human chromosome10q21-22. It is up regulated in gastric cancer tissue.

3条为新的EST序列,其中1条获得cDNA全长序列,具有完整的读码框,编码120个氨基酸,命名为gcI,定位于染色体10q21-22。

3. AmphiANT, was identified from the gut cDNA library of amphioxus B. becheri tisingtauense, consists of 1289 bps and contains an 897 bps open reading frame (ORF) corresponding to a deduced protein of 298 amino acids.

AmphiANT全长1289 bps,含有一个897bps的开放阅读框,编码一个298个氨基酸组成的蛋白质。

4. Its opening reading-frame(ORF) is 759bp, and encodes a 253 amino acid peptide.

cDNA序列为759bp,编码253个氨基酸,具有5个WD结构域。

5. Open reading'frame(ORF)

ORF

6. ORF 2 truncated gene

ORF2截短基因

7. ORF 75 gene

ORF75基因

8. Based on the result, three other ORFs orfG1, orfG2 and orfM encoding the glycosyltransferase and mannosyltransferase linked to orfE were obtained by subcloning and sequencing (Bankit number:476862; accession number: AY131228) .

orfG1，orfG2和orfM分别推测为葡萄糖转移酶基因和甘露糖转移酶基因(Bankit number：476862；收录号：AY131228)。

9. It contained an integrate ORF, which was 864 bp and encoded 287 amino acids that included 35 basic amino acids, 50 acidic amino acids, 95 hydrophobic amino acids and 60 polar amino acids.

ORF全长864bp，编码287个氨基酸，其中含有35个强碱型氨基酸（K、R），50个强酸型氨基酸（D、E），95个疏水氨基酸（A、I、L、F、W、V）和60个极性氨基酸（N、C、Q、S、T、Y）。

10. The identification of an ORF provides the first evidence that a new sequence of DNA is part or all of a gene encoding for a particular protein.

ORF的识别是证实一个新的DNA序列为特定的蛋白质编码基因的部分或全部的先决条件。

11. ORF) Gentlemen, we are talking so much about Michael.

ORF）先生们，我们谈论了许多有关迈克尔的事情。

12. The full-length cDNA of the OsBIRF1 is 1837 bp with a predicted 1191bp open reading frame (ORF), which predicts to encode a 396 amino acid protein containing all conserved domains of the RING finger protein.

OsBIRF1基因全长cDNA为1873bp,包含一个1191bp的ORF,编码一个含396个氨基酸的蛋白,并含有RING指蛋白所有的特征性保守结构域。

13. over-lapping ORF

overlapping open reading frame

14. The ORF encoding p10 was 261 nucleotide long and encoding a putative 87 amino acid polypeptide of 9.3 kD.

P10基因编码区全长261bp，可编码分子量为9.3kD，由87个氨基酸残基组成的多肽。

15. Initial determination of function for Pib gene ORF region by rice transformation

Pib基因编码区功能的转基因初步鉴定

16. No equivalent protein of Pns9 encoded by RGDV S9 ORF was found in other members of the genus Phythoreo-virus.

RGDV S9片段编码的Pns9蛋白在植物呼肠孤病毒属内未发现同源蛋白，其功能尚待确定。

17. Several bacterial genes had been reported, including ompA, ibeA, ibeB , and yijP relating to invasion of BBB.The yijP gene also calledf577 and ibe23 , has a 1,734 - nucle-otide ORF.

yijp基因，也称为f577或IBE23基因，其ORF为1734bp，推断的编码产物的分子量为66.6KD，该蛋白参与大肠杆菌侵袭BMEC的机制不清。

18. The ZFP28 cDNA sequence is 4104bp and contains a 2076-bp open reading frame (ORF) that encodes an 868 amino acid protein with an N-terminal signal peptide, two KRAB domains and 14 C-terminal C2H2 zinc finger motifs.

ZFP28长4104个碱基，含有一个长为2076个碱基的开放阅读框，编码一个长为868个氨基酸的蛋白质，它包含一个N-端信号肽，两个KRAB结构域和14个C-端C2H2型锌指结构。

19. coli was studied. In order to acquire gene engineering strains with high activity of aminotransferase, the primer of AspC was designed to obtained the ORF of gene aspC.

为了获得具有高活力天冬氨酸转氨酶的基因工程菌,本研究设计AspC 引物在大肠杆菌中获取了编码天冬氨酸转氨酶的基因aspC 的ORF。

20. The ORF of two circadian gene BMAL1 and CRY1 were cloned from normal human whole brain marathon cDNA and verified with restriction enzyme digestion and DNA sequencing.

从正常人全脑marathon cDNA中克隆到人生物节律基因BMAL1和CRY1的开放读码框架(ORF)，经酶切和测序鉴定与GeneBank数据库中收录的序列基本一致。

21. The CMO ORF was cloned and the plant expression vector pBI121-CMO was constructed. The resultant plasmids pBI121-CMO was transferred into Agrobacterium tumefaciens (GV3101) by the liquid nitrogen freezing thaw method.

克隆了CMO基因的编码区,构建了其植物表达载体pBI121-CMO,冻融法将质粒导入根癌农杆菌(Agrobacterium tumefaciens)GV3101,获得工程菌GV3101-pBI121-CMO。

22. The company uses the international freight forwarding control software and gives full play to the advantage orf internet to connect the customers net and the agency net closely at home and abroad .

公司使用国际货运管理系统软件,充分发挥互联网优势,将客户、国内外代理网络紧密相联。

23. Keywords human papillomavirus type 6 （HPV6）;PCR;L1 ORF;nucleotide sequence variation;

关键词人乳头瘤病毒6型;聚合酶链式反应;L1开放读码框;基因序列多态性;

24. ORF include of 633bp, encoding 210 amino acids with molecular 23.6 KDa weight and pI is 6.28. CGH peptide include 12.4% Basic Amino Acids, 11.9% Acidic Amino Acids, 32.4% Hydrophilic Amino Acids, 43.3% Hydrophonic Amino Acids.

分子量为23.6KD，等电点为6.28； 疏水氨基酸占43.3%，亲水氨基酸占32.4%，碱性氨基酸占12.4%，酸性氨基酸占11.9%。

25. "At first, I thought he was making a bad joke," Austrian broadcaster ORF quoted the woman, Helga Aichwalder, as saying.

受到威胁的妇女海尔加·艾克瓦尔德说：“一开始，我还以为他是在开一个恶劣的玩笑。”

26. Orf subgroup virus

口疮亚组病毒

27. Orf parapoxvirus

口疮副痘病毒

28. orf virus

口疮病毒, 接触传染性肱庖皮炎病毒, 羊痘病毒

29. open reading frame, ORF

可译框架, 阅读框架

30. open reading frame(ORF)

可读框, 可译框架, 开放阅读框架

31. There is terminate codon TAA of the whole ORF at the 3D coding region termination.

在3D编码区末端有整个ORF的终止密码子TAA。

32. Ten mutants of CCR5 ORF were identified, seven of which were non-synonymous.

在CCR5 ORF共检测到10个突变,其中7个为非同义突变;

33. This Web site shall not be considered ab solicitation for or offering of any investment product or service to anyiperson in any jurisdiction where such solicitation orf offering would be illegal.

在一些境外法律管辖范围认为诱导或提供任何投资产品或服务给任何人是违法的，这个网站则不能认为是在此区域诱导业务或提供任何投资产品或服务。

34. Application of Orf Teaching Method in Children's Accordion Class

奥尔夫教学法在儿童手风琴集体课中的运用

35. Olympus ORF;

奥林巴斯 ORF；

36. The genomes of Ningbo isolate (accession AJ851866) and Panan isolate (accession AJ885005) are all consisted of 9723 nucleotides (excluding the polyA tail) with a single large predicted ORF.

宁波分离物(序列登录号AJ851866)和磐安分离物(序列登录号AJ885005)的基因组均由9723个核苷酸组成(不包括polyA尾),均具有单一开读框。

37. Prisoner u wist on a familiar quote "If at first you don't succeed, give up" orf aw play on words or on a situation.

它可以是一句常言的歪曲如“你要是一开始不成功，就放弃”，或者是玩弄语言和情景。

38. The ORF was inserted into expression vector pSE-380, then transformed into E. coli JM109 and E.

将此ORF连接入质粒pSE-380中，并在大肠杆菌JM109和BL21中表达。

39. Keywords Nestin;ORF;expression;cytoskeleton;growth cone;P19 neurons;cerebellar granule cells;

巢蛋白;开放阅读框;表达;细胞骨架;生长锥;P19神经元;小脑颗粒细胞;

40. The full length cDNA of ANGPTL4 consists of 1943 nucleotides in which there is a 1218 bp ORF encoding a 406-amino acid polypeptide (predicted molecular mass is 45.2 kDa).

并用RACE-PCR获得其全长。该基因cDNA全长为1943 bp，开放阅读框含1218 bp，编码406个氨基酸，预测分子量为45.2 kDa。

41. Cloning of DHBV Complete Genome from Guangdong Brown Ducks and Finding of a New ORF

广东麻鸭DHBV全基因克隆及新读码框的发现

42. Opening reading fragment (ORF)

开放性读码框

43. open reading frame(ORF);

开放性阅读框;

44. An open reading frame (ORF) is a portion of a gene's sequence that contains a sequence of bases, uninterrupted by stop sequences, that could potentially encode a protein.

开放阅读框是基因序列的一部分，包含一段可以编码蛋白的碱基序列，不能被终止子打断。

45. Open reading frame (ORF)

开放阅读框（ORF）

46. Identification and Cloning of a Novel Full ORF Gene of P. Falciparum PDZ Domain Containing Protein

恶性疟原虫新的PDZ包含蛋白编码区基因的分离与克隆

47. We cloned the full length ORF of gene At2g31070 from Columbia wild type Arabidopsis. According to the result of bioimformatics analysis, At2g31070 has the conserved TCP domain so belongs to TCP family.

我们以哥伦比亚野生型拟南芥为模板得到基因At2g31070的全长ORF,生物信息学分析结果表明它具有TCP家族保守结构域,属于TCP家族的转录因子。

48. The Founder orf HKCOC used to be a pastor of methodist and you can see how great its effect is .

据他老人家说，当时有许多年轻人冒充蒙召前来报读，给他拒绝了。

49. Methods The pCI neo KH ORF expression vector was constructed by DNA recombinant technique and was introduced into COS 7 cells and K562 cells by lipofectactin mediated DNA transfection. Expression of KH ORF mRNA was detected by RT PCR.

方法 采用DNA重组技术构建KH ORF的表达载体pCI neo KH ORF ,通过脂质体法转染COS 7细胞和K5 6 2细胞 ,RT PCR检测目的基因的表达。

50. Analysis of Structure of ITS Sequence on Monocots with Reticulate Vein Basen ORF Finder

方法的植物ITS片段结构特点分析

51. The earliest Mother's Day celebrations can be traced back to the spring celebrations of ancient Greece in honor of Rhea,the Mother orf the Gods.

最早的母亲节可以追溯到古希腊，那时是为了纪念众神之母赫拉。

52. Surprisingly, some isolates accumulate to high levels but still can’t be detected by ELISA.Sequence analysis revealed distinctive amino acids changed in the coat protein ORF of these isolates.

本论文首先探讨酵素连结免疫抗体法准确度的限制因子，发现病毒含量过低或是鞘蛋白胺基酸序列有变异，皆为造成ELISA侦测准确度不高之原因。

53. According to the data of TMV MP in the GeneBank, design gene specific primer, amplified of the whole open reading frame (ORF).

根据GeneBank中TMV MP的序列设计基因特异引物，扩增出运动蛋白基因的整个开放阅读框（ORF）。

54. Each open reading frame (ORF) is knocked out using a PCR-based gene deletion strategy that takes advantage of the high degree of homologous recombination in yeast.

每个开放读码框使用基于PCR基因删除策略,使用在酵母中的高度同源重组优势。

55. Sequencing and bioinformatics analyses indicated that UB-39K gene consisted two integrated ORF, BmVUB and BmV39K.

测序和生物信息学分析表明,遍在蛋白-39K基因片段包括两个完整的开放阅读框(ORF):遍在蛋白(BmVUB)和39K(BmV39K);

56. ORF = owner's risk of fire

火险由货主负责

57. The points for attention in production of the orf virus living vaccine using bovine testis cells

用犊牛睾丸细胞生产羊口疮疫苗时应注意的问题

58. Objective To observe the protective effict of hepatitis E virus(HEV)ORF 2 recombinant protein expressed in prokaryote cell cynomolgus macaques(cynos)against challenging with wild-type HEV.

目的观察基因重组戊型肝炎病毒(HEV)结构蛋白对恒河猴戊型肝炎野病毒攻击的保护作用。

59. ORF Amplification of immobilization antigen from Ichthyophthirius multifiliis by degenerate PCR

简并PCR扩增小瓜虫抑动抗原基因的

60. Cloning of orf 224 gene correlated with cytoplasmic male sterility (CMS) and its plant expression vector construction

细胞质雄性不育相关基因orf224的克隆及其植物表达载体的构建

61. The recombinant palsmid(pGEX-4T-2/ IL-15) was obtained and sequence analysis verified its opening read fram(ORF) is correct.

经酶切和测序分析,表明成功构建了pGEX-4T-2/IL-15原核表达克隆;

62. Based on the position weight matrix(PWM) and length distribution of open reading frame(ORF),a simple method for predicting translation initiation sites is presented. It can identify TIS from upstream AUGs easily.

结合位置权重矩阵(PWM,position weight matrix)和开放阅读框架(ORF,open reading frame)的长度分布特征建立了简单的方法识别翻译起始位点,此方法能很好地区分上游AUG和TIS.

63. Results 1.Both GAP-43 ORF gene fragment and the recombinant AAV-EGFP-GAP-43 plasmid were verified by enzyme digestion and DNA sequencing for correct reading frame and orientation.

结果:. 1.克隆出的rGAP-43基因的ORF区片段以及构建的AAV-EGFP-rGAP-43重组表达质粒,两者经DNA序列测定,所测序列与GeneBank检索的完全一致。

64. The whole ORF of P10 and P17 protein genes of those 13 ARV strains were 297bp and 441bp in length, respectively.

结果显示,13个ARV毒株的P10蛋白基因ORF全长均为297bp,编码98个氨基酸;

65. The BC-48 cDNA was 610?bp in length with a 570?bp ORF encoding 189 amino acids. Similarity of nucleotide sequence of the BC-48 gene with the sequence from GenBank(U46551)was 96.7%.

结果显示,克隆的BC-48基因片段长610 bp,含有一个570 bp的开放阅读框,编码189个氨基酸,与GenBank中USDA株(U46551)的同源性为96.7%;

66. Results show that some codons' usage in synonymous codon repeat segments is different from that in the whole ORF sequences not all the codons' RSCU values are relative to the gene expression level.

结果表明部分密码子的使用与ORF全序列中的使用不同，并非所有密码子的使用与基因表达水平正相关；

67. The results show that our supposition is correct.The ratio of the number of known ORF and short theoretical ORF are given.The relation may be useful for gene identification.

给出了短的理论ORF中可能的编码序列所占比例的分布曲线，这对识别短的编码序列有参考价值。

68. Vettel told the ORF programme Sport am Sonntag: "But if the diffuser helps us, then it will also help in Monaco."

维特尔告诉编码方案体育报：“但是如果扩散帮助我们，那么它也将有助于在摩纳哥."

69. Isolation and Biological Characterization of Orf Virus

羊传染性脓疱病毒分离及生物学特性研究

70. sheep orf virus

羊口疮病毒

71. The isolation and identification of orf virus

羊口疮病毒的分离与鉴定

72. Furthermore, the impact of the P protein on ongoing translation of the C ORF was directly demonstrated by polysome distribution analysis.

而且，通过多核糖体分布分析可以直接表明P蛋白对C ORF翻译的影响。

73. The cDNA of vitellogenins in Philosamia cynthia ricini consists of 5788bp, one ORF is 5337 bases which codes 1779 am ino acids.

蓖麻蚕卵黄原蛋白cDNA是由5788pb构成,一个ORF为5337个碱基,编码了1779个氨基酸。

74. Orf virus disease

触染性深脓疱病毒病

75. The BADH and CMO ORF were obtained and the plant expression vector pCU1303-BADH, pCU1303-CMO were constructed.

设计带特定酶切位点的特异引物,分别克隆了麦冬BADH和CMO的编码区片段,构建了植物表达载体pCU1303-BADH和pCU1303-CMO。

76. Keywords Transfusion transmitted virus（TTV）;Open reading frame（ORF）;Cloning;Prokaryotic expression;

输血传播病毒;ORF;克隆;原核表达;

77. A and B fragments of CYP2J3 gene were connected by overlap extension PCR to obtain ORF sequence of CYP2J3 gene.

运用重叠延伸聚合酶链反应连接CYP2J3基因A和B两片段,扩增出大鼠CYP2J3基因全长ORF序列。

78. Using genome blast, MAGE-H1/Apr-1 was mapped to chromosome Xp11.22 with the open reading frame (ORF) locating in one exon.

进一步在人基因组数据库利用Blast比较的方法将MAGE-H1/Apr-1定位在Xp11.22。

79. The growth hormone cDNA gene (cgh) of grassfish (Ctenopharyngodon idellus) was obtained by RT-PCR, the sequence analysis showed that the ORF (open reading frame) of cgh gene, encoding 210 amino acid residences, was 633 nucleotides.

通过RT-PCR获得草鱼(Ctenopharyngodon idellus)生长激素cDNA基因(cgh),序列研究表明,cgh基因的ORF为633nt,编码210aa.

80. The spheroidin gene of Dociostarus kraussi EPV(DkEPV)was cloned,sequenced and analyzed. In the DkEPV spheroidin gene,a ORF of 2 922 bps was harbored that it was capable of encoding polypeptide of 109.4 kDa.

通过对红胫戟纹蝗痘病毒Dociostarus kraussiEPV(DkEPV)包涵体蛋白基因克隆、测序与同源性分析,结果显示,DkEPV包涵体蛋白基因包含2 922 bps的开放阅读框架,编码109.4 kDa蛋白质。