sephadex

sephadex

1. The BPS1 were further purified by a column chromatogram of DEAE-cellulose, Sephadex G-75 to get the BPS 111. Their homogeneity were proved by Sephadex G-100 chromatography.

BPS1再经DEAE-纤维素、Sephadex G-75凝胶柱层析得到BPS11；

2. DEAE Sephadex

Deae

3. The molecular mass of the PLC was determined as 64 kDa using SDS-PAGE, which was in agreement with the results obtained from Sephadex G-200 column chromatography (65 kDa), suggesting that the PLC was a monomeric protein.

SDS-PAGE电泳显示该磷脂酶C呈现单一条带,分子量约为64 kDa。 通过Sephadex G-200凝胶过滤层析柱标定的磷脂酶C的分子量为65 kDa,两者的结果一致,表明该酶是单体蛋白质。

4. Sephadex G-15

Sephadex

5. PROGESTERONE RECEPTOR ASSAY BY SEPHADEX LH20 CHROMATOGRAPHY A COMPARISON WITH DCC ASSAY

Sephadex LH20凝胶过滤法分离孕酮受体与DCC法比较

6. DEAE-Sephadex A50, Sephadex G-75 and CNBr activated Sephrose 4B were purchased from Pharmacia Corp.

主要试药：DEAE-Sephadex A50、Sephadex G-75、CNBr Activa-t。

7. The crude protein was obtained by extracting the leaves of Blechnum orientale with PBS and precipitating with (NH 4) 2SO 4. The crude protein was purified by Sephadex G-100 filtration after treatment through active carbon column.

乌毛蕨的叶片经pbs抽提和硫酸铵沉淀 ,得到的粗蛋白经活性碳柱处理后 ,在分子筛层析柱SephadexG -10 0上纯化得到一种凝集素 (BlechnumOrientaleLectin ,简称BOL) .

8. diethylaminoethanol sephadex technique

二乙氨基乙醇葡聚糖凝胶法

9. Sephadex is a group of highly specialized gelfiltration and chromatographic media which composed of macroscopic beads synthetically derived from the polysaccharide,dextran.

交联葡聚糖凝胶)是多糖类物质,尽管其结构、性能较稳定,但在压力、强酸、氧化剂存在情况下,也会发生部分糖苷键水解,或凝胶交联结构受损。

10. Keywords Sephadex gel adsorption application.;

交联葡聚糖凝胶;吸附;应用;

11. Keywords Sephadex;swelling;dehydration;drying;

交联葡聚糖凝胶;溶胀;脱水;干燥;

12. The Adsorption Behvior and Application of Sephadex gel

交联葡聚糖凝胶吸附性能及应用

13. sephadex column chromatography

交联葡聚糖柱色谱法

14. diethylaminoethyl Sephadex

交葡聚糖凝胶

15. lipophilic sephadex

亲脂性葡聚糖凝胶

16. amm-Horsfall glycoprotein (THP)was purified from human urine by repeated salting outfractination and Sephadex G-100 Chromatography.

人尿Tamm－Horsfall糖蛋白（THP）经盐析、SephadexG－100层析获得SDS－PAGE均一的蛋白质纯品。

17. The yield of crude HG dHG was 0.25%. Sephadex LH-20 column chromatogramphy was used to purify dHG.

以Sephadex LH-20凝胶对HG粗dHG进行进一步的分离纯化。

18. sephadex G-10

凝胶色谱法

19. The compare of physicochemistry characteristic of several standards of sephadex between national products and external products

几种国产与进口葡聚糖凝胶的比较

20. Purification of Insulin by CMC and Sephadex G50

利用CMC与Sephadex G50纯化胰岛素

21. The rapeseed peptides (RSP-R) ,produced with Alcalase and Flavourzyme, was separated by Sephadex G-25 gel filtration chromatography, and three parts of peptides (RSP-1RSP-2 and RSP-3) were obtained.

双酶分步水解的菜籽清蛋白粗品肽(RSP-R)经SephadexG-25凝胶层析柱分离纯化,得到分子量依次降低的三个级分(RSP-1,RSP-2和RSP-3)。

22. Takes a sample to Sephadex - the G75 column， collects the first peak， after the freeze drying obtains the white mPEG - SOD.

取样上Sephadex-G75柱，收集第一峰，冻干后获得白色的mPEG-SOD。

23. The most frequently used commercial gel is Sephadex.

商业上常用的凝胶是交联葡聚糖凝胶。

24. Xylanases purification from Aspergillus niger were studied.Three kinds of xylanases, X1, X2 and X3, were obtained by means of isolation from Sephadex G-100 and DEAE-Sephadex A-50.

对黑曲黴(Aspergillus niger)木聚糖酶进行了纯化研究，结果表明，经Sephadex G-100和DEAE-Sephadex A-50分离后，获得三个组分，称为X1、X2、X3。

25. BH-1 was isolated and purified from the fermentation broth by rough fractionation and Sephadex G-100 gel chromatography.

将发酵上清液经粗分级分离和Sephadex G-100凝胶过滤，得到具有纤溶活性的柱层析产物BH-1。

26. We selected BSM as expression medium, and BMP was purified by Sephadex G- 25 and then de- tected by RP- HPLC.

建立了SephadexG-25分离纯化、RP-HPLC检测BMP的有效方法。

27. We have chosen Sephadex G-75 as gel matrix, conducted the research about refolding of MT3-MMP and realized its refolding. 4.

我们使用Sepharose G-75作为复性凝胶介质，对金属螯合层析纯化的MT3-MMP进行了柱上复性研究，较好地实现了包涵体蛋白的复性。

28. Methods:Melittin was isolated and purified from bee venom by gel filtration on Sephadex G 25,Sephadex G 50 and Sephadex G 75.Homogenicity and molecular weight of melittin were determined by SDS PAGE and HPLC.

方法 :用 Sephadex G- 2 5、Sephadex G- 5 0、Sephadex G- 75三步层析法从蜜蜂毒中分离纯化蜂毒素 ,以SDS- PAGE定性 ,以 HPL C进行含量测定。

29. Method: The liposomes and dissociated drugs were separated by sephadex filtration,mini-column centrifugation and dialysis.

方法:分别采用凝胶过滤法、微柱离心法、透析法分离脂质体和游离药物,并进行方法学考察,优化包封率测定条件。

30. Mothods: The tannin in injections of TCM containing (SM) was separated from the water soluble active ingredients of SM with Sephadex gel.Then it was detected normally.

方法:利用葡聚糖凝胶分离丹参有效成分与鞣质,然后常规检查。

31. Methods Based on the selective sorption of Sephadex CM-C25 resin for vitamin B6, a renewable drop spectrofluorimetry was used for simultaneous determination of vitamin B2 and B6 in a mixed solution.

方法基于葡聚糖凝胶CM-C25对维生素B6的选择性吸附,采用液滴荧光分析方法对维生素B2和B6混合溶液进行检测。

32. The recombinant K99 and F41 fimbriae antigens expressed by E. coli RRI (pMG611) strain had been purified by heat treatment and then by Sephadex G-100 filtratiion.

本文利用加热搅拌及Sephadex G-100凝胶过滤方法,从双价重组工程菌RRI(pMG611)中分离了重组K99和F41菌毛抗原。

33. After a long process of experiment, finally isolate the active fraction from Flos Daturae for psoriasis with Sephadex LH-20 column chromatography and water-methol solvent system successly.

本文经过长期反复的摸索,最终采用葡聚糖凝胶(Sephadex LH-20)柱色谱以水-甲醇溶剂系统成功地对洋金花治疗银屑病的有效部位进行了分离。

34. Utilizing heat treatment and gel filtration chromatography on Sephadex G-150, antihypertensive factor (AHF) was partially purified from erythrocytes of essential hypertensive subjects.

本研究利用热处理和Sephadex G-150凝胶过滤层析等方法,从原发性高血压病患者(EHS)红细胞中部分纯化了抗高血压因子(AHF)。

35. Keywords polysaccharides of hemp;sephadex column chromatograph;molecular weight;monosaccharide;

火麻多糖;凝胶柱层析;分子量;单糖;

36. Keywords Polysaccharides of hemp;Sephadex column chromatograph;Isolation and Purification;Structure analysis;

火麻多糖;分离纯化;结构分析;

37. The fibrinolytic principle was obtained by twice DEAE-Sephadex A-50 column chromatography and Sephadex G-100 gel filtration from the venom of Agkistrodon halys Pallas.

用二乙氨基乙基葡聚糖A-50两次柱层析及葡聚糖凝胶过滤,从浙江产蝮蛇毒中得到一个纤溶组份。

38. To set up a sephadex column chromatography method for the determination of polymer impurity in cefotetan disodium.

目的建立葡聚糖凝胶色谱法测定头孢替坦二钠中聚合物的方法。

39. OBJECTIVE To determine the trap efficiency of liposomes using Sephadex.

目的考察用葡聚糖凝胶法测定脂质体包封率的影响因素。

40. Keywords Anemarrhena asphodeloides;Chemical Constituents steroidsponin;Mid-pressure Column;Sephadex;

知母;化学成分;甾体皂甙;中压柱;硅胶;

41. Surface Adsorpton Behavior of Heteroly Acid on Sephadex Gels

砷钼蓝杂多酸在葡聚糖凝胶表面吸附

42. Twoelution peaks were obtained on the Sephadex G100 column chromatography of the crude extractand the active substance was found in the first peak.

粗提液经 Sephadex G100层析后,得到了两个洗脱峰,活性物质存在于第一峰中。

43. Recently, the J6-1 membrane associated factor, stimulating proliferation of J6-1 cells, was discovered and designed as MAF-J6-1. After fractionation via Sephadex G-100 and sequential two steps FPLC Mono Q, it was purified over 333-fold.

经Sephadex G-100凝胶过滤及连续两次快速蛋白液相层析(FPLC,Mono Q)将其分离。

44. It shows that the PPMC-1 is homogeneous polysaccharide through Sephadex G-200 arbitration, acryl amide gel electrophoresis arbitration.

经Sephadex G-200等方法鉴定和理化分析表明PPMC-1为均一多糖。

45. Encapsualtion efficiencies of liposomes were measured by Sephadex chromatographic method and the shape of liposomes was observed by transmission electronic microscope.

结果:表面活性剂的种类和含量对脂质体的变形性有显著影响,表面活性剂的嵌入改变了脂质体的理化性质及包封率。

46. The EtOH fraction was concentrated and loaded on a Sephadex LH 20 column eluted with 0.5 L of H 2O to obtain 15 fractions, which were detected by sili...

结论:黄芩苷苷元是主要药物代谢产物的中间体,他们在体内共存,构成黄芩苷的药效。

47. Keywords 10-hydroxycamptothecin;Semisolid lipid nanoparticles（SSLN）;Sephadex gel;

羟基喜树碱;半固体脂质纳米粒;葡聚糖凝胶;

48. Keywords liposomes and nanoliposomes;encapsulation efficiency;protamine aggregation method;sephadex chromatography method;

脂质体和纳米脂质体;包封率;鱼精蛋白凝聚法;葡聚糖凝胶柱色谱法;

49. The enzyme was purified by batch-wise adsorption and elution on DEAE-cellulose, Butyl-Toyopearl 650M hydrophobicchromatography and Sephadex G-100 gel filtration chromatography, The purityof the enzyme was increased by 66.8-fold.

脂酶提纯时,采用DEAE-纤维素搅拌吸附与洗脱、Bulyl-Toyopearl650 M疏水层析及交联葡聚糖G-100凝胶过滤层析工艺,可提高酶纯度66.8倍。

50. STUDY ON RADIATION GRAFTING OF NASI ON SEPHADEX AND CONJUGATION OF THE COPOLYMER WITH BSA

葡聚糖与 NASI 辐射接枝共聚及与 BSA 的偶合反应

51. sephadex gel

葡聚糖凝胶

52. Keywords sephadex gel;heteropoly acid;adsorptiol;

葡聚糖凝胶;杂多酸;吸附;

53. Keywords Sephadex;column layer analysis;liposomes;trap efficiency;

葡聚糖凝胶;柱色谱法;脂质体;包封率;

54. sephadex G

葡聚糖凝胶G

55. Sephadex LH-20

葡聚糖凝胶LH-20

56. sephadex microsphere

葡聚糖凝胶微球[栓塞治疗用]

57. sephadex chromatogram

葡聚糖凝胶柱

58. Keywords sephadex column chromatography;cefotetan disodium;polymer;

葡聚糖凝胶色谱;头孢替坦二钠;聚合物;

59. Sephadex filtration

葡聚糖凝胶过滤法

60. Sephadex G10

葡萄糖凝胶G10

61. Keywords Sephadex G10;Sulbenicillin sodium;High molecular weight impurities;

葡萄糖凝胶G10;磺苄西林钠;高分子杂质;

62. Keywords polysaccharides of Spirulina;platensis;sephadex column chromatography;elution;

螺旋藻多糖;凝胶柱层析;洗脱;

63. In this paper,the authors introduced a kind of available Sephadex recovery and preservation method-- dehydrating/drying method that could greatly improve the stability and repeat utilization of Sephadex.

该文根据作者自己的工作经验,着重介绍了回收后的交联葡聚糖的干燥保存法,以提高交联葡聚糖的稳定性和重复使用率。

64. The ultrafiltration and chromatographic experiments on Sephadex G-200 showed the range and the distribution of molecular weight.

超过滤和葡聚糖凝胶G 200实验,显示了其分子量的范围和分布。

65. FA and FC were further isolated by Sephadex G75, and FA-1, FA-2, FC-1 and FC-2 were collected.

进一步用Sephadex G75分离FA和FC,得FA-1、FA-2、FC-1和FC-2。

66. A protease purified from the fresh leaves of Acer truncatum Bunge by means of Sephadex A-50, Sephadex G-200 column chromatography.

采用Sephadex A-50,Sephadex G-200柱层析的方法,从槭树科槭树属元宝枫(AcertruncatumBunge)的叶片中提取并纯化了叶蛋白酶.

67. A total of 20 compounds were isolated from the strain F121112 by the column chromatography on silica gel, RP-Cig and Sephadex LH-20, followed by reverse-phase high performance liquid chromatography.

采用生物活性跟踪分离的方法,通过硅胶柱层析、ODS C-18柱层析、Sephadex LH-20凝胶层析以及RP-HPLC等各种色谱技术,对F121112产生的生物活性物质进行分离纯化,得到了20个化合物。

68. It was efficient to use Sephadex G 50 column chromatography and ultra violet absorption spectrometry atomic absorption spectrophotometry in isolation, and then it was followed by quantitative analysis of chromium binding substances.

采用葡聚糖凝胶G?50柱层析法分离低分子蛋白效果好。

69. Keywords sinomenine liposomes;entrapment efficiency;sephadex filtration;mini-column centrifugation;dialysis;

青藤碱脂质体;包封率;凝胶过滤法;微柱离心法;透析法;